Enhanced leachate phytodetoxification test combined with plants and rhizobacteria bioaugmentation

Plant combination and rhizobacterial bioaugmentation are the modification of constructed wetlands (CWs) to promote the detoxification of leachate. In this study, characterization of leachate was carried out to ensure the maximum concentration of leachate that did not affect the plant's growth. Herein, the identification of leachate-resistant rhizobacteria is used to determine the type of bacteria that is resistant and has the potential for leachate processing in the next step. The phytodetoxification test is carried out by comparing the addition of rhizobacteria and without the addition of rhizobacteria to detox leachate parameter Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), Total Suspended Solid (TSS), Total Nitrogen (TN), Cadmium (Cd), and Mercury (Hg). Results showed that used plants could still live in the largest leachate concentration of 100%. The rhizobacteria that were identified and bioaugmented in the reactor were Bacillus cereus, Nitrosomonas communis, and Pseudomonas aeruginosa. Phytodetoxification test by a single plant showed the efficiency ranged between 40% and 70%. The addition of rhizobacterial bioaugmentation and plant combination can improve the percentage of COD 80.47%, BOD 84.05%, TSS 80.05%, TN 75.58%, Cd 99.96%, and Hg 90%. These modifications are very influential for leachate detoxification through plant uptake and rhizodegradation processes.