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Cell-Selective Multifunctional Surface Covalent Reconfiguration Using Aptamer-Enabled Proximity Catalytic Labeling.

Yuna Guo^1,2, Nan Wang¹, Yihong Zhong¹

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Journal of the American Chemical Society

|February 23, 2023

Summary

Researchers developed a new cell surface engineering platform using aptamers and catalysis for precise, multifunctional cell labeling. This method enables simultaneous installation of multiple molecules, allowing for complex cell programming and tracking in mixed populations.

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Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

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Published on: September 20, 2016

Area of Science:

Biotechnology
Chemical Biology
Cell Biology

Background:

Cell surface engineering is crucial for creating custom cell interfaces with specific functions.
Existing cell-selective covalent labeling methods struggle to simultaneously introduce multiple distinct functional molecules.

Purpose of the Study:

To develop an aptamer-enabled proximity catalytic covalent labeling platform for multifunctional cell surface reconfiguration.
To enable selective and simultaneous installation of multiple functional molecules on target cells within mixed populations.

Main Methods:

Conjugation of peroxidase enzymes with cell-selective aptamers to create targeted labeling probes.
Utilizing proximity-induced catalysis for site-specific covalent labeling of target cells.
Employing phenol-modified functional molecules for diverse cell surface manipulations and multiplexed labeling.

Main Results:

Demonstrated selective covalent labeling of target cells in mixed populations.
Achieved multiplexed covalent labeling, simultaneously installing two orthogonal click reactive molecules.
Successfully performed ultrafast neoglycosylation on living cells, enabling subsequent glycan remodeling.

Conclusions:

The aptamer-enabled platform provides a versatile tool for multifunctional cell surface engineering.
This method allows for precise control over cell-cell interactions and surface remodeling in complex biological systems.
The platform offers a novel approach for cell-selective glycocalyx engineering.