Related Experiment Video
Updated: Aug 9, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Reflective axicon based energy-efficient extended depth of focus quasi-Bessel beam probe for common-path optical
Abstract:
This work presents an optical fiber negative/reflective axicon probe that generates an energy-efficient quasi-Bessel beam (QBB) having a central spot (CS) possessing ∼20% of the QBB power. With silver coating around the axicon, the CS power has been increased by ∼45%. The QBB possesses a large depth of field, ∼400µm, with a micron order spot size as obtained experimentally. The probe has further been explored for common-path optical coherence tomography. The probe length has been optimized to minimize the path length difference between the reference and sample signal. With a divergence angle of just 0.013°, the beam provides a lateral resolution of ∼2.5 to ∼16µm for an axial distance of 0.1 to 1.0 mm. The imaging results are presented for standard samples such as onion and Scotch tape.
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

