An improved rapid method for DNA recovery from cotton swabs
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Alexander Gray1, Agnieszka Kuffel1, Niamh Nic Daeid1
1Leverhulme Research Centre for Forensic Science, University of Dundee, Dundee, UK.
Abstract:
We present a novel rapid method for the recovery of cellular and free DNA from cotton swabs based on a simple elution buffer containing a high molecular weight polymer and detergent combined with a short proteinase K digestion to release cellular DNA. This method shows increased yields approaching 80% recovery of the input DNA compared to the QIAamp DNA Mini kit standard extraction protocol for swabs which has a recovery of 20-30%. The buffer components in the described method are compatible with direct PCR analysis of the isolated DNA without further purification. Recovery efficiencies were estimated by qPCR.

