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Related Experiment Video

Updated: Aug 9, 2025

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
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Immunocapturing rare cells from blood: A simple and robust microsystem approach.

Jiří Smejkal1, Petr Aubrecht1, Alena Semerádtová1

  • 1Centre for Nanomaterials and Biotechnology, Faculty of Science, Jan Evangelista Purkyně University in Ústí nad Labem, 400 96, Ústí nad Labem, Czech Republic.

Biosensors & Bioelectronics
|February 23, 2023
PubMed
Summary

This study introduces a novel stop-flow microsystem for efficient capture of rare cells. The technology achieves up to 90% efficiency, aiding early disease diagnostics.

Keywords:
Cell immunocaptureCirculating endothelial cellsCirculating tumor cellsMicrosystemsRare cell population

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Biology

Background:

  • Circulating rare cells (CRCs) are crucial biomarkers for early disease detection and prognosis.
  • Existing methods for CRC isolation face challenges in efficiency and specificity.

Purpose of the Study:

  • To develop and validate a simple, robust stop-flow microsystem for effective immunocapture of rare cells.
  • To investigate factors influencing cell immunocapture efficiency in a microfluidic device.

Main Methods:

  • Fabrication of a stop-flow microsystem using glass microblasting and 3D printing.
  • Design of a planar antibody-coated channel for immunocapture.
  • Monitoring cell capture efficiency via fluorescence microscopy.

Main Results:

  • Achieved up to 90% immunocapture efficiency for MCF-7 cells in whole blood using CD326 antibody-coated surfaces.
  • Demonstrated the effectiveness of the stop-flow dynamic regime for rare cell isolation.
  • Analyzed the impact of surface modifications, flow conditions, and medium complexity on capture efficiency.

Conclusions:

  • The developed stop-flow microsystem offers a promising approach for rare cell isolation and quantification.
  • The findings provide insights for designing advanced microsystems for cell-size-independent rare cell analysis.
  • This technology has potential applications in medical diagnostics and prognostics.