Robustness and stability of acetate-driven partial denitrification (PD) in response to high COD/NO₃^--N

Partial Denitrification (PD) producing nitrite for anammox may face the issue of relatively high chemical oxygen demand (COD) loading (i.e., COD/NO₃^--N) due to real wastewater being changed in substrate concentration and flowrate. In this study, three PD systems (R1, R2, R3) with sodium acetate providing electrons were developed to investigate the influence of the relatively high COD/NO₃^--N ratios (4.0, 6.0, and 8.0) on NO₂^--N production and the subsequent recoverability. It was found that a relatively high NO₂^--N production with nitrate-to-nitrite transformation ratio (NTR) of 74.0% could be still obtained despite COD/NO₃^--N even improving to 8.0 under limited reaction time (10 min) with small nitrate remaining. However, a deteriorated nitrite production was observed with sufficient reaction time (15 min) with NTR being lowered to 19.2%. Delightedly, when reducing influent COD/NO₃^--N to a normal level of 3.0, PD with high nitrite production was rapidly achieved after suffering from a relatively high COD/NO₃^--N (4.0-8.0) for 130 cycles. Besides, it was found the relatively high COD/NO₃^--N had a minor influence on the recoverability of PD, as evidenced by the close NTRs. Microbial analysis revealed the relative abundance of PD functional bacteria, Thauera, decreased under high COD/NO₃^--N, while it is still highly dominated in the systems, varying from 75.1% in R1 to 62.8% in R3 after around 110-cycles recovery. Furthermore, it appeared that the high pH (9.1-9.2) induced by sodium acetate also likely played a role in maintaining the excellent PD. Overall, this study demonstrated the robustness and stability of acetate-driven PD in response to high COD/NO₃^--N, further informing the technological superiority of PD in supplying stable and efficient nitrite, which provided solid technical support to apply it with anammox for high-efficient N removal.