Related Experiment Video
Updated: Aug 9, 2025
06:11
Culturing Primary Rat Inner Medullary Collecting Duct Cells
Published on: June 21, 2013
14.9K
Aquaporin-2 is not alone
1Research Area Cardiovascular & Metabolic Diseases, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany; DZHK (German Center for Cardio vascular Research), Partner Site Berlin, Germany.
Kidney International
|February 23, 2023
Summary
Yes-associated protein (YAP) maintains expression of aquaporin water channels in kidney collecting duct cells. This discovery clarifies how these cells regulate water balance under resting conditions.
Area of Science:
- Nephrology
- Molecular Biology
- Cell Biology
Background:
- Arginine-vasopressin regulates water reabsorption in kidney collecting ducts via aquaporins (AQPs) 2, 3, and 4.
- Maintaining AQP expression is crucial for short-term plasma osmolality adjustments.
- The mechanism controlling AQP expression in principal cells under basal conditions was previously unknown.
Purpose of the Study:
- To elucidate the mechanism responsible for maintaining aquaporin expression in kidney collecting duct principal cells under resting conditions.
Main Methods:
- Investigated the role of transcription coregulators in regulating AQP expression.
- Focused on the yes-associated protein (YAP) and its potential involvement.
Main Results:
- Identified YAP as a key regulator for the coordinated expression of aquaporins 2, 3, and 4.
- Demonstrated YAP's critical role in maintaining AQP expression under basal conditions.
Conclusions:
- Yes-associated protein (YAP) is essential for the constitutive expression of aquaporins 2, 3, and 4 in collecting duct principal cells.
- This finding provides a novel molecular mechanism for regulating water reabsorption and maintaining fluid balance.
More Related Videos
Related Concept Videos
01:25Aquaporins
4.9K
Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
4.9K
01:17Pore Transport and Ion-Pair Transport
551
Pore transport and ion-pair formation are critical mechanisms for the absorption and distribution of drugs in the body.
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct...
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct...
551
01:45Endocrine Signaling
64.6K
Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
64.6K
01:30ATP Driven Pumps III: V-type Pumps
3.8K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
3.8K
01:26Reabsorption and Secretion in the DCT and Collecting Duct
1.5K
The early phase of the DCT manages the reabsorption of approximately 10-15% of filtered water, 5–10% of filtered sodium, and 5–10% of filtered chloride. This process is facilitated by Na+–Cl− symporters in apical membranes and sodium-potassium pumps, as well as Cl− leakage channels in basolateral membranes. The early DCT also stands out as a site where parathyroid hormone (PTH) stimulates calcium reabsorption, depending on the body's requirements.
The distal...
The distal...
1.5K
01:27ATP Driven Pumps I: An Overview
8.3K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.3K