Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.2K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reinnervated Split-Muscle Technique for Creating Additional Myoelectric Sites in an Animal Model.

Plastic and reconstructive surgery·2016
Same author

Graphene oxide-based efficient and scalable solar desalination under one sun with a confined 2D water path.

Proceedings of the National Academy of Sciences of the United States of America·2016
Same author

Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration.

Stem cell research & therapy·2016
Same author

Nanotechnology Based Green Energy Conversion Devices with Multifunctional Materials at Low Temperatures.

Recent patents on nanotechnology·2016
Same author

State-Dependent Allosteric Inhibition of the Human SLC13A5 Citrate Transporter by Hydroxysuccinic Acids, PF-06649298 and PF-06761281.

Molecular pharmacology·2016
Same author

Designing therapeutic cancer vaccine trials with delayed treatment effect.

Statistics in medicine·2016

Related Experiment Video

Updated: Aug 9, 2025

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.9K

Transcript annotation of Chinese sturgeon (Acipenser sinensis) using Iso-seq and RNA-seq data.

Xiaolin Liao1, Libin Zhang2, Hua Tian3

  • 1Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem, Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan, 430079, China. xliao@mail.ihe.ac.cn.

Scientific Data
|February 23, 2023
PubMed
Summary

This study provides the first comprehensive genomic and transcriptomic data for the critically endangered Chinese sturgeon. These findings offer crucial genetic insights for conservation efforts aimed at protecting this vulnerable species.

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.7K
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.3K

Related Experiment Videos

Last Updated: Aug 9, 2025

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.9K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.7K
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.3K

Area of Science:

  • Aquatic Biology
  • Genomics
  • Conservation Genetics

Background:

  • The Chinese sturgeon (Acipenser sinensis) is critically endangered, primarily inhabiting the Yangtze River.
  • Conservation strategies often involve artificial propagation, but genomic data is scarce.

Purpose of the Study:

  • To perform full-length transcriptomic sequencing of Chinese sturgeon.
  • To identify long non-coding RNAs (lncRNAs) and coding sequences (CDS).
  • To analyze gene expression profiles and differentially expressed genes (DEGs) across tissues.

Main Methods:

  • PacBio Iso-seq and RNA-seq were employed on 10 Chinese sturgeon tissues.
  • Cluster analysis of gene families and phylogenetic analysis of 14 species were conducted.
  • Identification of lncRNAs, CDS, and analysis of gene expression profiles.

Main Results:

  • 19,538 full-length transcripts were obtained, ranging from 51 bp to 7,033 bp.
  • lncRNAs and CDS were identified in all analyzed tissues.
  • Gene expression profiles and DEGs were analyzed across the 10 tissues.

Conclusions:

  • This study provides valuable full-length transcript sequences and gene expression profiles for Chinese sturgeon.
  • The generated data will support future functional genomic studies and aid in understanding the genetic basis of endangerment.
  • These resources are vital for developing effective conservation strategies for the Chinese sturgeon.