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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
Published on: February 9, 2011
First report of Staphylococcus pseudintermedius ST71-SCCmec III and ST45-ΨSCCmec57395 from canine pyoderma in
Mariela E Srednik1, Claudia A Perea2, Gabriela I Giacoboni3
1National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA, U.S.A.. mariela.srednik@gmail.com.
Abstract:
Staphylococcus pseudintermedius is an opportunistic pathogen commonly associated with skin infections in dogs. Twenty-three methicillin-resistant S. pseudintermedius (MRSP) isolated in Argentina from dogs with pyoderma were analyzed using whole genome sequencing (WGS) and classified into sequence types (ST) by multilocus sequence typing (MLST) and staphylococcal chromosome cassette mec (SCCmec) types.Based on the WGS analysis, MLST, and SCCmec type results, we report for the first time in Argentina two MRSP strains, one each, belonging to ST71-SCCmec III and ST45-ΨSCCmec57395 from dogs with pyoderma. We also identified seven isolates with ST339, which had been previously reported in only two isolates in Argentina. Additionally, we identified ten MRSP isolates harboring variants of the SCCmec V found in S. aureus, seven SCCmec V (5C2&5) with two ccrC1 recombinases, and three SCCmec V (5C2) with one ccrC1 recombinase.Our findings provide important insights into the evolution and geographic spread of these hypervirulent dominant clones that threaten the health of our companion animals and represent a significant risk for zoonotic infections.
Insights
Two novel methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains were identified in Argentina. This study enhances understanding of MRSP evolution and spread in canine pyoderma, posing zoonotic risks.
Area of Science:
- Veterinary Microbiology
- Genomics
- Infectious Diseases
Background:
- Staphylococcus pseudintermedius is a common cause of canine pyoderma.
- Methicillin-resistant S. pseudintermedius (MRSP) poses a significant threat to animal and human health.
- Understanding the genetic diversity and geographic distribution of MRSP is crucial for control.
Purpose of the Study:
- To characterize methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains isolated from dogs with pyoderma in Argentina.
- To identify novel sequence types (ST) and staphylococcal chromosome cassette mec (SCCmec) types of MRSP in the region.
- To investigate the genetic relatedness and potential for zoonotic transmission of dominant MRSP clones.
Main Methods:
- Whole genome sequencing (WGS) was performed on 23 MRSP isolates.
- Multilocus sequence typing (MLST) was used to determine sequence types.
- SCCmec typing was conducted to classify the mobile genetic elements carrying methicillin resistance.
Main Results:
- Two MRSP strains, ST71-SCCmec III and ST45-ΨSCCmec57395, were identified for the first time in Argentina.
- Seven isolates of ST339 were identified, a type previously reported only twice in Argentina.
- Ten MRSP isolates harbored variants of SCCmec V, including subtypes with differing numbers of ccrC1 recombinases.
Conclusions:
- This study reports the first identification of ST71-SCCmec III and ST45-ΨSCCmec57395 MRSP in Argentina.
- The findings highlight the emergence and spread of specific hypervirulent MRSP clones in canine populations.
- These MRSP strains represent a potential risk for zoonotic infections and underscore the need for ongoing surveillance.
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