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Updated: Aug 9, 2025

Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography
Published on: September 2, 2020
X-ray micro-tomographic data of live larvae of the beetle Cacosceles newmannii
Philipp Lehmann1,2, Marion Javal1, Anton Du Plessis3
1Centre for Invasion Biology, Department of Conservation Ecology and Entomology, Stellenbosch University, Stellenbosch, South Africa.
Abstract:
Quantifying insect respiratory structures and their variation has remained challenging due to their microscopic size. Here we measure insect tracheal volume using X-ray micro-tomography (μCT) scanning (at 15 μm resolution) on living, sedated larvae of the cerambycid beetle Cacosceles newmannii across a range of body sizes. In this paper we provide the full volumetric data and 3D models for 12 scans, providing novel data on repeatability of imaging analyses and structural tracheal trait differences provided by different image segmentation methods. The volume data is provided here with segmented tracheal regions as 3D models.
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