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Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

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Updated: Aug 9, 2025

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Published on: August 10, 2016

Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation.

Catarina Almeida¹, Augusto Q Pedro¹, Ana P M Tavares¹

¹CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal.

Frontiers in Bioengineering and Biotechnology

|February 24, 2023

Summary

This summary is machine-generated.

Ionic liquids offer a promising solution for biopharmaceutical manufacturing, potentially reducing high downstream processing costs and enhancing the stability of complex biological drugs. This review explores their advancements.

Keywords:

biomanufacturing biopharmaceuticals biopharmaceuticals administration downstream processing formulation ionic liquid-based approaches ionic liquids purification platforms

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Area of Science:

Biopharmaceutical Manufacturing
Biotechnology
Chemical Engineering

Background:

Biopharmaceuticals, including proteins, nucleic acids, peptides, and vaccines, have transformed medicine.
Downstream processing constitutes over 80% of biopharmaceutical production costs, hindering accessibility.
Formulation is critical for maintaining therapeutic efficacy and stability of biological products.

Purpose of the Study:

To review advancements in ionic-liquid applications for biopharmaceutical downstream processing.
To explore the use of ionic liquids as excipients for stable biopharmaceutical formulations.
To critically analyze progress and provide a SWOT analysis of ionic-liquid-based approaches.

Main Methods:

Literature review of ionic-liquid applications in biopharmaceutical purification.
Analysis of ionic liquids in formulation development for enhanced stability.
SWOT analysis of ionic-liquid technologies in biopharmaceutical production.

Main Results:

Ionic liquids show potential for improving purity and recovery yields in downstream processing.
Ionic liquids can serve as effective excipients, enhancing the stability of biopharmaceutical formulations.
Current research indicates significant promise for ionic liquids in reducing manufacturing costs and improving product quality.

Conclusions:

Ionic-liquid-based strategies present a viable and innovative approach to overcome key challenges in biopharmaceutical manufacturing.
Further research and development are warranted to fully realize the potential of ionic liquids in this field.
Ionic liquids offer a dual benefit of cost reduction and enhanced product stability for biopharmaceuticals.