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Updated: Aug 16, 2026
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Published on: March 10, 2017
A micromethod for in vitro assay of lymphocyte proliferation in mouse whole blood
Abstract:
A microplate culture system to assay the in vitro proliferative responses of mouse peripheral blood lymphocytes to phytohaemagglutinin is described. The method utilises whole blood, a gamma-emitting isotopic label and semi-automated harvesting. Data are presented to show the effects of several variables on the culture system. The technique is suitable for serial study of the in vitro phytohaemagglutinin response in individual animals.
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