Related Experiment Video

Updated: Aug 9, 2025

Modeling Colitis-Associated Cancer with Azoxymethane AOM and Dextran Sulfate Sodium DSS
12:37

Modeling Colitis-Associated Cancer with Azoxymethane AOM and Dextran Sulfate Sodium DSS

Published on: September 11, 2012

47.1K

Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of

Ahmed Aj Jabbar1, Ibrahim Abdel Aziz Ibrahim2, Fuad O Abdullah3,4

  • 1Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil 44001, Iraq.

Current Issues in Molecular Biology
|February 24, 2023
PubMed

Insights

Onosma mutabilis extract shows anticancer effects against colon cancer cells in vitro. In vivo, it protects against azoxymethane-induced colon cancer in rats by reducing aberrant crypt foci and inflammation.

Area of Science:

  • Pharmacology
  • Cancer Biology
  • Natural Products Chemistry

Background:

  • Onosma species are recognized for their medicinal properties.
  • Colorectal cancer remains a significant global health concern.
  • Investigating natural compounds for cancer prevention is crucial.

Purpose of the Study:

  • To evaluate the in vitro anticancer and in vivo chemoprotective effects of Onosma mutabilis extract (OME).
  • To assess OME's efficacy against azoxymethane (AOM)-induced aberrant crypt foci (ACF) in a rat model.

Main Methods:

  • In vitro antiproliferative activity against Caco-2 and HT-29 cells using MTT assay.
  • In vivo studies involved biochemical analyses, oxidative stress and inflammatory biomarker assessment, ACF quantification, histopathology, and immunohistochemistry (Bax, Bcl-2).

Main Results:

  • OME exhibited antitumor activity against colorectal cancer cell lines (22.28-36.55 µg/mL).
  • OME supplementation significantly reduced ACF formation and modulated Bax/Bcl-2 protein expression in AOM-treated rats.
  • OME improved antioxidant status (SOD, CAT, GPx) and potentially regulated the Nrf2-Keap1 pathway.

Conclusions:

  • Onosma mutabilis extract demonstrates significant chemoprotective potential against AOM-induced colon cancer in rats.
  • OME's efficacy may stem from its antioxidant, anti-inflammatory, and pro-apoptotic properties.
  • Further research into O. mutabilis as a chemopreventive agent is warranted.
Keywords:
Boraginaceaechemopreventioncolon cancerhistopathologyoxidative stress

More Related Videos

Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice09:01

Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice

Published on: March 10, 2015

10.1K
Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer06:24

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

1.0K

Related Concept Videos

Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.3K
Drugs for Treatment of Ulcerative Colitis in IBD01:29

Drugs for Treatment of Ulcerative Colitis in IBD

Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
209