Diosmin-Loaded Nanoemulsion-Based Gel Formulation: Development, Optimization, Wound Healing and Anti-Inflammatory...

Area of Science:

• Biomaterials Science
• Dermatology
• Nanotechnology

Background:

• Chronic wounds pose a significant clinical challenge due to complex healing processes.
• Engineered biomaterials offer a promising strategy for advanced wound care.
• Diosmin (DSM) is a flavonoid with potential therapeutic properties for wound healing.

Purpose of the Study:

• To formulate and characterize a novel diosmin (DSM)-loaded nanoemulsion (NE)-based gel.
• To evaluate the wound healing and anti-inflammatory efficacy of the developed DSM-loaded NE gel in a rat model.

Main Methods:

• Nanoemulsions (NEs) loaded with diosmin (DSM) were prepared using lauroglycol 90, Tween-80, and transcutol-HP.
• Formulations were optimized using Box-Behnken Design, focusing on globule size, PDI, and %EE.
• The optimized NE formulation (F1) was incorporated into a carbopol 940 gel for further evaluation.

Main Results:

• Optimized DSM-loaded NEs exhibited a globule size of 41 ± 0.07 nm, PDI of 0.073 ± 0.008, and %EE of 87 ± 0.81%.
• The F1-loaded gel showed favorable characteristics including drug content and spreadability.
• In vitro release studies and in vivo assessments demonstrated significant wound-healing and anti-inflammatory activities (p < 0.05).

Conclusions:

• A stable and effective diosmin-loaded nanoemulsion gel formulation was successfully developed.
• The nanoemulsion gel formulation significantly promotes wound healing and exhibits anti-inflammatory effects.
• This formulation represents a promising therapeutic approach for managing cutaneous wounds.