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Copper Traces Quantification in Bee's Products by Solid Surface Fluorescence. A Green Analytical Proposal.

Maria Carolina Talio1,2, Vanesa Muñoz3,4, Mariano Acosta3,5

  • 1INQUISAL-CONICET, Almirante Brown 907 (CP: D5700HHW), San Luis, Argentina. mcarolinatalio@gmail.com.

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Summary

A novel fluorescence method quantifies copper(II) using o-phenanthroline and eosin dyes on Nylon membranes. This sensitive technique is ideal for analyzing copper in bee products, offering accurate results validated by ICP-MS.

Keywords:
Bees productsCopper tracesEosinSolid surface fluorescenceo-Phenanthroline

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Area of Science:

  • Analytical Chemistry
  • Environmental Chemistry

Background:

  • Accurate quantification of trace metals like copper(II) is crucial for environmental and food safety monitoring.
  • Existing methods may lack selectivity or require complex sample preparation.

Purpose of the Study:

  • To develop a sensitive and selective solid-surface fluorescence (SSF) method for copper(II) determination.
  • To apply the developed method for analyzing copper in bee products.

Main Methods:

  • Utilized a ternary complex of copper(II) with o-phenanthroline (o-Phen) and eosin (Eo) dyes.
  • Employed solid-surface fluorescence (SSF) after selective retention of the complex on Nylon membranes.
  • Optimized experimental variables including reagent concentrations and retention conditions.

Main Results:

  • Achieved a low limit of detection (LOD) of 1.18 ng L⁻¹ and a limit of quantification (LOQ) of 3.57 ng L⁻¹.
  • Demonstrated adequate tolerance to common interfering species.
  • Successfully applied the method to determine copper in bee products from Argentina, with recovery rates near 100%.

Conclusions:

  • The proposed SSF methodology offers a sensitive, selective, and robust approach for copper(II) quantification.
  • The method is environmentally friendly and suitable for analyzing copper in complex matrices like bee products.
  • Validation by ICP-MS confirmed the accuracy and reliability of the developed analytical technique.