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Updated: Jun 29, 2026

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Single-Molecule Sizing through Nanocavity Confinement.

Raphaël P B Jacquat^1,2, Georg Krainer¹, Quentin A E Peter¹

¹Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, United Kingdom.

|February 24, 2023

Summary

This summary is machine-generated.

This study introduces nanocavity diffusional sizing (NDS), a new method to measure the size of nanoscale particles and biomolecules in solution. NDS uses particle residence times in nanocavities to determine hydrodynamic radii, enabling precise sizing for various applications.

Keywords:

biosensing confocal detection microfluidics nanofluidics protein sizing single molecules

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Area of Science:

Nanotechnology
Biophysics
Analytical Chemistry

Background:

Accurate sizing of nanoscale particles and biomolecules is crucial for fields like nanobiotechnology and clinical diagnostics.
Existing sizing methods can be complex or lack single-molecule resolution.

Purpose of the Study:

To develop a novel, rapid, and quantitative method for sizing nanoscale particles and single biomolecules in solution.
To establish nanocavity diffusional sizing (NDS) as a viable technique for particle characterization.

Main Methods:

Theoretical modeling and simulations to understand particle diffusion within nanocavities.
Experimental demonstration using single-molecule confocal microscopy to measure particle residence times.
Utilizing nanofluidic cavities for controlled confinement of particles.

Main Results:

Particle residence times in nanocavities correlate with their diffusion coefficients and thus their sizes.
Demonstrated linear scaling of residence times with the size of colloids, protein aggregates, and DNA oligonucleotides.
NDS provides quantitative size measurements at the single-molecule level.

Conclusions:

Nanocavity diffusional sizing (NDS) is a novel optofluidic approach for precise particle sizing.
The method is applicable to a range of nanoscale entities, including biomolecules.
NDS holds significant potential for applications in nanobiotechnology, biophysics, and diagnostics.