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Updated: Aug 9, 2025

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
Digital colorimetric sensor for assessment of iodate in food-grade salt based on paper-based analytical device
Henley F Galiga1, Fortunato B Sevilla2
1The Graduate School, University of Santo Tomas, Manila 1015, Philippines; College of Arts and Sciences, Romblon State University, Romblon 5505, Philippines.
Abstract:
A digital colorimetric sensor was developed for the on-site measurement of iodate in food-grade salt. It involved a paper-based analytical device (PAD) containing an immobilized iodide/starch reagent and a smartphone as the detection system. Iodate ions produce a blue-black coloration on the PAD, the smartphone captures a digital image of the PAD, and an installed mobile app performs an analysis to generate color values related to the intensity of the color in the PAD. Parameters in the image acquisition and PAD preparation were optimized, and a matrix-matched calibration plot was employed for quantification. The plot exhibited a linear response in the concentration range of 10 to 100 mg iodate kg-1 of salt, and a detection limit of 2.20 mg kg-1 was determined. The present method was applied to analyze real samples, and the results agreed well with those obtained using the reference method at the 95% confidence level.
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