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Glycopeptide Capture for Cell Surface Proteomics
Published on: May 9, 2014
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Selective enrichment of glycopeptides using ground eggshell materials
Zehu Xie1, Shuwen Hua2, Shun Zhang3
1Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China; The Institute of Life and Health Science, University of Chinese Academy of Sciences, Ningbo, Zhejiang 315010, China.
Journal of Chromatography. A
|February 24, 2023
Summary
Researchers utilized natural eggshell for glycopeptide enrichment, offering a cost-effective and efficient alternative to complex synthetic materials in proteomics analysis.
Area of Science:
- Proteomics
- Glycobiology
- Biomaterials
Background:
- Protein glycosylation analysis relies on glycopeptide enrichment.
- Existing methods often involve complex synthesis, high costs, and extensive reagents.
- A need exists for simpler, more economical enrichment strategies.
Purpose of the Study:
- To investigate the use of natural eggshell for glycopeptide enrichment.
- To evaluate the efficiency and selectivity of eggshell in capturing glycopeptides.
- To explore eggshell as a sustainable material for glycosylation proteomics.
Main Methods:
- Eggshell was employed as a natural material for glycopeptide enrichment.
- Horseradish peroxidase (HRP) tryptic digest was used as a standard sample.
- Performance was assessed based on sensitivity, selectivity, size-exclusion, loading capacity, and recovery.
Main Results:
- Eggshell demonstrated high sensitivity (0.05 fmol μL⁻¹), good selectivity (1:1000 ratio), and excellent size-exclusion (1:10,000 ratio).
- It exhibited a loading capacity of 75 mg g⁻¹ and a recovery rate of 97.6 ± 0.3%.
- 153 and 114 glycopeptides were successfully captured from human serum digests of normal and diabetic individuals, respectively.
Conclusions:
- Natural eggshell is an effective and environmentally friendly material for glycopeptide enrichment.
- Its unique porous structure and biomass facilitate efficient capture and separation of glycopeptides.
- Eggshell presents a promising, low-cost alternative for glycosylation proteomics analysis.
