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Nonlethal Blood Sampling from the Killifish Nothobranchius furzeri.

Luca Dolfi1, Roberto Ripa1, Danel Medelbekova1

  • 1Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany.

Cold Spring Harbor Protocols
|February 24, 2023
PubMed
Summary

This study presents a minimally invasive blood collection protocol for the African turquoise killifish, enabling repeated sampling for aging research. The method allows for longitudinal studies and reduces the number of animals needed.

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Area of Science:

  • Comparative physiology
  • Animal models for aging research
  • Laboratory animal science

Background:

  • Blood withdrawal is crucial for monitoring fish health and physiology.
  • Nonlethal blood collection supports longitudinal studies and reduces animal use.
  • The African turquoise killifish is a valuable model for aging research due to its short lifespan.

Purpose of the Study:

  • To develop and detail a minimally invasive protocol for repeated blood sampling in the African turquoise killifish.
  • To address the challenges of blood collection in small-bodied fish models.

Main Methods:

  • A detailed protocol for repeated blood sampling from individual African turquoise killifish was established.
  • The method was tested on fish aged 6 to 24 weeks.
  • Blood extraction volumes were recorded based on specimen age, sex, and size.

Main Results:

  • The protocol enables minimally invasive, repeated blood sampling from the same fish.
  • Blood volumes ranging from 0.5 to 8 µL were successfully extracted.
  • The extracted blood volume is sufficient for various analyses, including glucose measurement and cell counts.

Conclusions:

  • This protocol facilitates longitudinal studies in the African turquoise killifish, a key model for aging research.
  • The method is minimally invasive and does not cause lasting harm.
  • It supports essential physiological and metabolic analyses in this emerging model organism.