Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational assembly of synthetic marine biofilm community with chitinase production.

Research square·2026
Same author

Exploring the functional composition of the human microbiome using a hand-curated microbial trait database.

BMC bioinformatics·2021
Same author

Phosphotransferase System Uptake and Metabolism of the β-Glucoside Salicin Impact Group A Streptococcal Bloodstream Survival and Soft Tissue Infection.

Infection and immunity·2020
Same author

Isolation and characterization of diverse microbial representatives from the human skin microbiome.

Microbiome·2020
Same author

Trait-based analysis of the human skin microbiome.

Microbiome·2019
Same author

Detecting interaction networks in the human microbiome with conditional Granger causality.

PLoS computational biology·2019
Same journal

Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

Biotechnology and bioengineering·2026
Same journal

Glycoengineered Recombinant Alpha1-Antitrypsin Results in Comparable In Vitro and In Vivo Activities to Human Plasma-Derived Protein.

Biotechnology and bioengineering·2026
Same journal

Minimizing Off-Target Effects of CRISPR-Cas9 With Optimized sgRNA: Evaluation of Efficiency and Specificity in the Tumor Protein 53 (TP53) Region.

Biotechnology and bioengineering·2026
Same journal

Metabolic Flux Analysis Reveals Cell Line-Specific Rewiring in CHO Cells Following TCA Cycle Intermediate Feeding for Bioprocess pH Control.

Biotechnology and bioengineering·2026
Same journal

Photohydrogenotrophic Cultivation of Purple Non-Sulfur Bacteria in an Open Bioreactor: Enhanced Selectivity Through Light Cycling and Ammonium Limitation.

Biotechnology and bioengineering·2026
Same journal

Translating Blue Light Stimulation From Batch to Perfusion: Process and Intracellular Metabolic Analysis.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: Aug 9, 2025

High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health
11:40

High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health

Published on: April 28, 2022

2.8K

Screening microbiota for effects on host tissues.

Andrea C Timm1, Bryan Brensinger1, Meaghan Hart1

  • 1Research and Exploratory Development Department, Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland, USA.

Biotechnology and Bioengineering
|February 25, 2023
PubMed
Summary
This summary is machine-generated.

Microbial secretions impact host cells, influencing community assembly. A new microfluidic device enables scalable screening of these effects on skin cells, revealing toxicity insights.

Keywords:
cytotoxicitymicrofluidicsskin microbiome

More Related Videos

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
08:14

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

Published on: February 28, 2018

8.9K
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
08:33

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

13.1K

Related Experiment Videos

Last Updated: Aug 9, 2025

High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health
11:40

High-Throughput Screening of Microbial Isolates with Impact on Caenorhabditis elegans Health

Published on: April 28, 2022

2.8K
A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
08:14

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

Published on: February 28, 2018

8.9K
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
08:33

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

13.1K

Area of Science:

  • Microbiology
  • Cell Biology
  • Bioengineering

Background:

  • Microbial community assembly is influenced by environmental factors and organismal metabolism.
  • Microbial secretions mediate interactions, impacting host cells both positively and negatively.
  • Understanding these secretions is crucial for insights into microbiome function and potential interventions.

Purpose of the Study:

  • To investigate the functional impact of microbial secretions, specifically focusing on toxicity.
  • To examine the morphological effects of microbial secretions on skin-associated host cells.
  • To develop scalable methods for analyzing microbial secretion effects due to the complexity of microbial communities and host tissues.

Main Methods:

  • Quantification of supernatant dosage responses using image data analysis.
  • Development and demonstration of a microfluidic device for high-content screening.
  • Assessment of morphological changes in adherent skin-associated host cells exposed to microbial secretions.

Main Results:

  • Characterization of dose-dependent morphological effects of microbial secretions on host cells.
  • Demonstration of a microfluidic platform's capability for high-content screening of secretion impacts.
  • Establishment of a method to quantify toxicity and other effects of microbial secretions.

Conclusions:

  • Microbial secretions have discernible morphological effects on host cells, including toxicity.
  • The developed microfluidic device provides a scalable solution for high-content screening of microbial secretions.
  • This approach offers key mechanistic insights into microbiome function and potential therapeutic strategies.