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Related Experiment Videos

Contractile response in isolated human groin lymphatics.

T Sjöberg1, P Alm, K E Andersson

  • 1Department of Surgery, Lund University, Sweden.

Lymphology
|September 1, 1987
PubMed
Summary

Human superficial groin lymphatics lack sympathetic nerves and alpha-adrenergic receptors. However, certain prostanoids, like prostaglandin F2 alpha and U44069, significantly induce lymphatic contractions, suggesting their role in lymphatic function.

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Area of Science:

  • Vascular Biology
  • Lymphatic Physiology
  • Pharmacology

Background:

  • Human superficial groin lymphatics are crucial for fluid balance but their neural and contractile regulation remains poorly understood.
  • Previous studies suggest potential roles for various vasoactive substances in lymphatic contractility.

Purpose of the Study:

  • To investigate the presence of sympathetic innervation in human superficial groin lymphatics.
  • To determine the contractile responses of these lymphatics to neurotransmitters and prostanoids.
  • To explore potential differences in lymphatic contractility in a patient with lymphedema.

Main Methods:

  • In vitro examination of human superficial groin lymphatic segments from 21 patients.
  • Histochemical analysis for catecholamine fluorescence and specific enzyme immunoreactivity (tyrosine hydroxylase, dopamine beta-hydroxylase).

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  • Isometric tension recording in tissue baths to assess contractile responses to K+, acetylcholine, amines (noradrenaline, adrenaline, dopamine, serotonin), and prostanoids (prostaglandin E2, prostaglandin F2 alpha, U44069).

    • Main Results:

    • No evidence of sympathetic nerves was found using histochemical methods.
    • Noradrenaline, adrenaline, and dopamine elicited weak or no contractile effects.
    • Serotonin induced contractions in some segments, while prostaglandin F2 alpha and U44069 caused significant concentration-dependent contractions.
    • Lymphatic tissue from the lymphedema patient showed a slightly enhanced response to noradrenaline and serotonin.

    Conclusions:

    • Human superficial groin lymphatics appear to lack sympathetic innervation and alpha-adrenergic receptors.
    • Specific prostanoids, particularly prostaglandin F2 alpha and U44069, are potent stimulators of human lymphatic contractility.
    • Prostanoids may play a significant role in regulating the contractile function of human superficial lymphatics.