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Updated: Aug 9, 2025

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Antimicrobial Polymeric Surfaces Using Embedded Silver Nanoparticles.
Pooja Sharma1,2, Luisa Fialho3,4, Nuno Miguel Figueiredo1
1CEMMPRE, Mechanical Engineering Department, University of Coimbra, 3030-788 Coimbra, Portugal.
Antimicrobial surfaces embedding silver nanoparticles (Ag NPs) show significant potential in preventing pathogen transmission. This cost-effective, scalable method enhances polymer surfaces, inhibiting bacterial growth on contact.
Area of Science:
- Materials Science
- Nanotechnology
- Microbiology
Background:
- Pathogens transmit diseases via contaminated surfaces, necessitating effective antimicrobial solutions.
- Antimicrobial surfaces offer a promising strategy to mitigate pathogen spread.
Purpose of the Study:
- To develop and evaluate antimicrobial polymer surfaces embedded with silver nanoparticles (Ag NPs).
- To assess the antibacterial efficacy of Ag NP-embedded polycarbonate (PC) against common bacteria.
Main Methods:
- Sputter deposition of a silver nanolayer onto a polycarbonate (PC) substrate.
- Two-step thermal annealing process to promote Ag NP diffusion, growth, and surface embedding.
- Evaluation of antibacterial capacity against Gram-positive and Gram-negative bacteria.
Main Results:
- Successful embedding of silver nanoparticles (Ag NPs) into the PC surface confirmed by height variation after annealing.
- Ag NP-embedded PC surfaces demonstrated significant inhibition of both Gram-positive and Gram-negative bacterial growth.
- The surface engineering process proved to be cost-effective and highly scalable.
Conclusions:
- Embedding antimicrobial Ag NPs into polymer surfaces is an effective strategy to create self-disinfecting materials.
- This method offers a scalable and economical approach to reduce pathogen transmission from surfaces.
- The developed antimicrobial surfaces hold potential for widespread application in public health and hygiene.
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