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Simple HPLC-UV Method for Piperacillin/Tazobactam Assay in Human Plasma
Khaled Abdelkawy1, Tyson Le2, Sherif Hanafy Mahmoud2
1Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh 6860404, Egypt.
Antibiotics (Basel, Switzerland)
|February 25, 2023
Summary
A new high-performance liquid chromatography (HPLC) method allows for sensitive and simultaneous quantification of piperacillin (Pip) and tazobactam (Taz) in plasma. This method supports therapeutic drug monitoring (TDM) for optimizing antibiotic dosing in critical care settings.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Piperacillin/tazobactam (Pip/Taz) is crucial for treating severe infections in ICUs.
- Therapeutic drug monitoring (TDM) of piperacillin may enhance treatment efficacy.
- A need exists for a reliable method to quantify Pip and Taz in plasma.
Purpose of the Study:
- To develop and validate a sensitive and simple HPLC method.
- To enable simultaneous quantification of piperacillin and tazobactam in human plasma.
- To support therapeutic drug monitoring (TDM) for Pip/Taz.
Main Methods:
- HPLC with UV detection at 218 nm.
- C18 column with gradient elution using an acetonitrile:water mobile phase.
- Simple sample preparation involving acetonitrile deproteinization.
Main Results:
- The method demonstrated excellent linearity (r² > 0.99) for both drugs.
- Acceptable precision, accuracy (within ±20%), and stability were achieved.
- Quantification ranges were 0.5-400 μg/mL for Pip and 1-100 μg/mL for Taz.
Conclusions:
- The developed HPLC method is sensitive, simple, and practical.
- The method's straightforward sample preparation and elution make it suitable for Pip/Taz TDM.
- Facilitates dose optimization for improved patient outcomes in critical care.
