Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phases of Wound Repair01:28

Phases of Wound Repair

6.1K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
6.1K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

9.1K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.1K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MLN51 (Metastatic Lymph Node Gene 51)/CASC3 (Cancer Susceptibility Candidate Gene 3), A Putative Tumour Suppressor in Breast Cancer, the Clinical and Therapeutic Connections.

Breast cancer (Dove Medical Press)·2026
Same author

[Corrigendum] Epithelial protein lost in neoplasm‑α (EPLIN‑α) is a potential prognostic marker for the progression of epithelial ovarian cancer.

International journal of oncology·2026
Same author

[Retracted] Knockdown of human antigen R reduces the growth and invasion of breast cancer cells <i>in vitro</i> and affects expression of cyclin D1 and MMP‑9.

Oncology reports·2026
Same author

Correction: Lin et al. Silencing CTNND1 Mediates Triple-Negative Breast Cancer Bone Metastasis via Upregulating CXCR4/CXCL12 Axis and Neutrophils Infiltration in Bone. <i>Cancers</i> 2021, <i>13</i>, 5703.

Cancers·2025
Same author

Tight junctional protein family, Claudins in cancer and cancer metastasis.

Frontiers in oncology·2025
Same author

[Corrigendum] Metastasis suppressor 1 expression in human ovarian cancer: The impact on cellular migration and metastasis.

International journal of oncology·2025
Same journal

Correction: Reis et al. Bioinks Enriched with ECM Components Obtained by Supercritical Extraction. <i>Biomolecules</i> 2022, <i>12</i>, 394.

Biomolecules·2026
Same journal

Correction: Kim, K.-H.; Yoo, B.C. Gintonin as a Lysophosphatidic Acid-Enriched GPCR Ligand System: Molecular Architecture and Receptor Pharmacology in <i>Panax ginseng</i>. <i>Biomolecules</i> 2026, <i>16</i>, 465.

Biomolecules·2026
Same journal

Correction: Bastyte et al. The Association of Vitamin D Receptor Gene Polymorphisms with Vitamin D, Total IgE, and Blood Eosinophils in Patients with Atopy. <i>Biomolecules</i> 2024, <i>14</i>, 212.

Biomolecules·2026
Same journal

AtHSPR Plays a Positive Role in Arabidopsis Resistance Against <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000 by Interacting with TOP1.

Biomolecules·2026
Same journal

CYTH4 Facilitates Renal Cell Carcinoma via Enhancing Proliferation and Likely Immune Evasion.

Biomolecules·2026
Same journal

Integrated Immune-Gut Profiling Identifies an Exploratory Pediatric Inflammatory Intestinal Profile Associated with Food-Specific IgG Reactivity.

Biomolecules·2026
See all related articles

Related Experiment Video

Updated: Aug 9, 2025

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.
09:06

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

Published on: March 25, 2020

8.8K

nWASP Inhibition Increases Wound Healing via TrKb/PLCγ Signalling.

Bethan A Frugtniet1, Fiona Ruge1, Andrew J Sanders2

  • 1Division of Cancer and Genetics, Cardiff University School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.

Biomolecules
|February 25, 2023
PubMed
Summary
This summary is machine-generated.

Neural Wiskott-Aldrich syndrome protein (nWASP) is elevated in chronic wounds. Inhibiting nWASP promotes wound healing by affecting cell behavior and signaling pathways, suggesting it as a therapeutic target.

Keywords:
chronic woundsmotilitynWASPtherapywound healing

More Related Videos

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
06:36

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

Published on: August 21, 2020

12.5K
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
09:34

Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

Published on: September 26, 2019

13.4K

Related Experiment Videos

Last Updated: Aug 9, 2025

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.
09:06

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

Published on: March 25, 2020

8.8K
Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
06:36

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

Published on: August 21, 2020

12.5K
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
09:34

Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

Published on: September 26, 2019

13.4K

Area of Science:

  • Wound Healing Research
  • Cellular Biology
  • Molecular Medicine

Background:

  • Chronic wounds pose a significant clinical and economic burden.
  • Identifying novel therapeutic targets for chronic wound healing remains a critical challenge.
  • The role of neural Wiskott-Aldrich syndrome protein (nWASP) in wound healing requires further investigation.

Purpose of the Study:

  • To evaluate nWASP as a potential therapeutic target for human wound healing.
  • To determine the regulatory mechanisms of nWASP in the context of wound repair.
  • To assess the impact of nWASP modulation on cellular functions essential for healing.

Main Methods:

  • Analysis of nWASP expression in clinical chronic wound cohorts.
  • Utilizing cell models (HaCaT and HECV) to study nWASP's influence on cellular functions.
  • Employing nWASP knockdown and specific inhibitors (wiskostatin, 187-1) in vitro.
  • Investigating the effects of nWASP inhibition on TrkB and PLCγ1 signaling pathways.
  • Evaluating nWASP inhibitor efficacy in a diabetic murine wound healing model.

Main Results:

  • nWASP transcript levels were significantly higher in human non-healing chronic wounds compared to healing tissues.
  • nWASP inhibition or knockdown altered the behavior of HaCaT and HECV cells.
  • TrkB signaling and downstream PLCγ1 phosphorylation were impaired by nWASP inhibition in HaCaT cells.
  • Treatment with an nWASP inhibitor significantly accelerated wound healing in a diabetic murine model.

Conclusions:

  • nWASP activity is associated with the non-healing phenotype of chronic wounds.
  • Modulation of nWASP impacts key cellular functions and signaling pathways (TrkB, PLCγ1) involved in wound repair.
  • nWASP represents a promising therapeutic target for non-healing chronic wounds.