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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Shotgun Lipidomics of Rodent Tissues
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Applied Clinical Tandem Mass Spectrometry-Based Quantification Methods for Lipid-Derived Biomarkers, Steroids and

Isabelle Matias1, Ilaria Belluomo1, Pierre-Louis Raux1

  • 1INSERM, Neurocentre Magendie, University of Bordeaux, U1215, F-33000 Bordeaux, France.

Biomolecules
|February 25, 2023
PubMed
Summary

New methods quantify steroids and cannabinoids in human plasma using tandem mass spectrometry. While mostly successful, these GLP-qualified methods are suitable for routine preclinical and clinical drug discovery studies.

Keywords:
GC-MS/MSLC-MS/MScannabinoidshuman plasmaquantification methodssteroidstandem mass spectrometryvalidation

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Area of Science:

  • Analytical Chemistry
  • Metabolomics
  • Mass Spectrometry

Background:

  • Metabolomics and quantification methods are crucial for identifying drug discovery biomarkers.
  • Tandem mass spectrometry (MS/MS) is a key technique requiring rigorous validation for clinical and preclinical use.
  • Steroids and cannabinoids, important lipid mediators, show potential interactions in addiction and metabolic disorders.

Purpose of the Study:

  • To develop and validate GC-MS/MS and LC-MS/MS methods for routine quantification of steroids and cannabinoids in human plasma.
  • To assess the suitability of these methods for drug discovery and related clinical applications.

Main Methods:

  • Gas chromatography-tandem mass spectrometry (GC-MS/MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were employed.
  • Isotopic approaches were used for method development and validation, including linearity, selectivity, LLOQ, matrix effect, carryover, accuracy, precision, and stability.
  • Quantification of 11 steroids and 7 cannabinoids in human plasma was performed.

Main Results:

  • The developed methods demonstrated satisfactory performance for most validation parameters.
  • Not all analytes met every acceptance criterion for all validity conditions.
  • Methanol proved more suitable than biological or surrogate matrices for calibration curves of endogenous compounds.

Conclusions:

  • The methods are considered GLP-qualified, suitable for routine analytical studies in preclinical and clinical settings.
  • Appropriate system suitability testing and quality controls in biological matrices are essential for reliable results.
  • These validated methods advance the quantification of steroids and cannabinoids for drug discovery research.