Amine Functionalized Gadolinium Oxide Nanoparticles-Based Electrochemical Immunosensor for Cholera.

Area of Science:

• Nanomaterials Science
• Biosensor Technology
• Analytical Chemistry

Background:

• Vibrio cholerae toxin (CT) poses a significant public health threat, necessitating sensitive and rapid detection methods.
• Gadolinium oxide nanoparticles (Gd2O3 NPs) offer unique electrochemical properties suitable for biosensor development.
• Existing CT detection methods may lack the sensitivity, speed, or cost-effectiveness required for widespread application.

Purpose of the Study:

• To synthesize and characterize Gd2O3 NPs for fabricating a highly efficient immunosensor.
• To functionalize Gd2O3 NPs and develop an electrochemical immunoelectrode for CT detection.
• To evaluate the performance of the developed immunosensor in terms of sensitivity and detection limit.

Main Methods:

• Microwave irradiation technique for Gd2O3 NP synthesis.
• Characterization using X-ray diffraction, transmission electron microscopy, and spectroscopy.
• Functionalization with APTES, electrophoretic deposition on ITO, and antibody immobilization via EDC-NHS chemistry.

Main Results:

• Successfully synthesized and characterized Gd2O3 NPs.
• Fabricated an APTES-Gd2O3/ITO immunoelectrode modified with anti-CT antibodies.
• Achieved a high electrochemical response to CT with a sensitivity of 8.37 mA ng-1 mL cm-2 and a detection limit of 1.48 ng mL-1.

Conclusions:

• The developed Gd2O3 NP-based immunosensor is highly efficient for CT detection.
• The straightforward synthesis and functionalization process offer a promising platform for biosensor development.
• This technology has the potential for rapid and sensitive diagnosis of cholera.