CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from...

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Materials Science

Background:

• Accurate glucose monitoring is crucial for diabetes management.
• Existing glucose detection methods can be time-consuming or lack sensitivity.
• Development of rapid, selective, and sensitive glucose probes is needed.

Purpose of the Study:

• To design and develop a ratiometric fluorescence probe for selective and rapid glucose detection.
• To optimize the probe's performance for a fixed, short detection time.
• To evaluate the probe's applicability in real-world samples like blood.

Main Methods:

• Conjugation of peroxyfluor-NHS (PF) with carbon dots (CDs) to create a Förster Resonance Energy Transfer (FRET)-based probe.
• Utilizing glucose oxidase (GOx) to produce H₂O₂, which reacts with PF.
• Developing a fixed 3-minute detection protocol based on kinetic studies of the PF-H₂O₂ reaction.

Main Results:

• The probe exhibited a ratiometric response with emission peaks at 450 nm and 517 nm.
• A linear relationship was observed between glucose concentration (0.05–5 mM) and the fluorescence intensity ratio (I₅₁₇/I₄₅₀) within a 3-minute detection time.
• The limit of detection (LOD) was determined to be 17.19 μM.
• Successful verification of the probe's applicability in blood glucose detection.

Conclusions:

• The developed CDs-PF probe allows for rapid and selective glucose detection.
• The fixed 3-minute detection method enhances efficiency without compromising linearity or sensitivity.
• This probe shows significant potential for point-of-care blood glucose monitoring applications.