Quantitative Analysis of Bioluminescence Optical Signal
Kazuki Niwa1, Hidehiro Kubota2, Toshiteru Enomoto2
1National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305-8563, Japan.
Biosensors
|February 25, 2023
Summary
Bioluminescence (BL) measurements are transitioning from relative to absolute values. This enables standardized quantification for reliable bioanalysis and bioreporter applications.
Area of Science:
- Biochemistry
- Molecular Biology
- Bioimaging
Background:
- Bioluminescence (BL) is light emission from luciferin-luciferase reactions in organisms.
- BL signals are used in bioanalysis, gene expression, and various bioimaging techniques.
- Current BL measurements are relative, limiting precise quantification.
Purpose of the Study:
- To highlight the shift towards absolute quantification of bioluminescence signals.
- To emphasize the importance of standardized measurements in bioanalysis and bioreporter fields.
- To introduce the concept of absolute optical signal measurements for biological events.
Main Methods:
- Review of recent approaches for absolute bioluminescence quantification.
- Determination of quantum yield for BL reactions.
- Photon counting of BL signals at the single-cell level.
Main Results:
- Development of methods for complete quantification of BL signals.
- Redetermination of BL reaction quantum yields.
- Enabling single-cell level photon counting for BL signals.
Conclusions:
- Absolute quantification of bioluminescence provides reliable and reproducible data.
- Standardized optical signal measurements are crucial for advancing bioanalysis and bioreporter applications.
- International Organization for Standardization (ISO) standards are key for reproducible biological event understanding.
