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Quantum Dot-Sensitised Estrogen Receptor-α-Based Biosensor for 17β-Estradiol.

Abongile N Jijana1,2, Usisipho Feleni3, Peter M Ndangili4

  • 1Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg, Johannesburg 2125, South Africa.

Biosensors
|February 25, 2023
PubMed
Summary

A novel biosensor utilizing estrogen receptor-α (ER-α) and tin selenide quantum dots detects 17β-estradiol (E2) in environmental samples. This highly selective sensor offers a crucial tool for monitoring E2 contamination in wastewater and environmental pollution management.

Keywords:
17β-estradiol (E2)SnSeendocrine-disrupting compounds (EDC)estrogen receptor-alpha (ER-α)hormonenanomaterialsquantum dots

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • 17β-estradiol (E2) is a potent estrogenic endocrine-disrupting compound (e-EDC) found in environmental water systems, posing significant health risks.
  • Accurate determination of E2 levels is critical for effective wastewater treatment and environmental pollution management.

Purpose of the Study:

  • To develop a highly selective and sensitive biosensor for the determination of 17β-estradiol (E2).
  • To leverage the specific binding affinity between estrogen receptor-α (ER-α) and E2 for sensor development.

Main Methods:

  • Fabrication of an electroactive sensor platform using a gold disk electrode functionalized with 3-mercaptopropionic acid-capped tin selenide (SnSe-3MPA) quantum dots.
  • Immobilization of estrogen receptor-α (ER-α) onto the SnSe-3MPA/AuE platform via amide chemistry to create the ER-α/SnSe-3MPA/AuE biosensor.
  • Electrochemical detection of E2 using square-wave voltammetry (SWV).

Main Results:

  • The developed ER-α/SnSe-3MPA/AuE biosensor demonstrated a formal potential of 217 ± 12 mV for E2 monitoring.
  • The biosensor exhibited a dynamic linear range (DLR) of 1.0-8.0 nM (R² = 0.99) and a limit of detection (LOD) of 1.69 nM.
  • High selectivity for E2 and good recovery rates were observed in milk samples, indicating practical applicability.

Conclusions:

  • The ER-α/SnSe-3MPA/AuE biosensor provides a sensitive and selective method for E2 detection.
  • This biosensor is a promising tool for environmental monitoring and wastewater treatment applications.
  • The study highlights the potential of quantum dot-based biosensors for detecting endocrine-disrupting compounds.