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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Progress in Fluorescence Biosensing and Food Safety towards Point-of-Detection (PoD) System.

Saloni Kakkar1,2, Payal Gupta2, Navin Kumar2

  • 1Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Chandigarh 160036, India.

Biosensors
|February 25, 2023
PubMed
Summary
This summary is machine-generated.

This review highlights fluorescence-based nanomaterials and microfluidic biosensors for rapid, sensitive, and user-friendly detection of foodborne pathogens. These advanced systems offer improved on-site food screening capabilities for public health and environmental safety.

Keywords:
PoC devicebiosensingfluorescence microscopyfood pathogenmicrofluidic

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Environmental Science

Background:

  • Foodborne pathogen detection is critical for public health and environmental safety.
  • Conventional detection methods face limitations in sensitivity and speed.
  • Nanomaterials offer superior sensitivity and selectivity for fluorescent-based detection.

Purpose of the Study:

  • To review the application of fluorescence-based nanomaterials in biosensors for pathogen detection.
  • To summarize recent advancements in integrated biosensor systems, including microfluidics and paper-based devices.
  • To evaluate the potential of these systems for on-site food screening.

Main Methods:

  • Review of literature on fluorescence-based nanomaterials and biosensor technologies.
  • Analysis of integrated microsystems, DNA probes, antibodies, and trapping components.
  • Evaluation of paper-based lateral-flow test strips and microchips.
  • Assessment of portable device performance for food screening.

Main Results:

  • Nanomaterials enhance sensitivity and selectivity in fluorescent detection compared to organic dyes.
  • Microfluidic biosensors are advancing towards sensitive, inexpensive, user-friendly, and rapid detection.
  • Integrated systems incorporating nanomaterials, probes, and antibodies show promise.
  • Paper-based devices and microchips are suitable for portable applications.

Conclusions:

  • Fluorescence-based nanomaterial biosensors represent a significant advancement in foodborne pathogen detection.
  • Integrated microfluidic systems and portable devices are key for on-site food screening.
  • Future development should focus on enhancing performance for real-time, on-site pathogen stratification.