A Multiple-Array SPRi Biosensor as a Tool for Detection of Gynecological-Oncological Diseases

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Oncology

Background:

• Multiplexed biomarker detection in body fluids enhances diagnostic accuracy.
• Current diagnostic methods may lack the capacity for simultaneous analysis of multiple key biomarkers.

Purpose of the Study:

• To develop and validate a multiple-array Surface Plasmon Resonance imaging (SPRi) biosensor for simultaneous detection of five cancer biomarkers.
• To assess the sensor's performance and applicability in real patient samples for early disease detection.

Main Methods:

• Development of a single SPRi chip integrating five individual antibody-functionalized gold biosensors.
• Covalent immobilization of antibodies (anti-CA125, anti-HE4, anti-CEA, anti-IL-6, anti-aromatase) using NHS/EDC chemistry on a cysteamine-modified gold surface.
• Testing and validation using patient plasma samples from ovarian cancer and endometrial cyst cases.

Main Results:

• The multiple-array SPRi biosensor successfully detected CA125, HE4, CEA, IL-6, and aromatase simultaneously.
• Detection ranges were suitable for clinical application: IL-6 (pg mL⁻¹), CA125 (µg mL⁻¹), and others (ng mL⁻¹).
• High precision was achieved: CA125 (3.4%), HE4 (3.5%), CEA (5.0%), IL-6 (5.0%), and aromatase (7.6%).

Conclusions:

• The developed multiple-array SPRi biosensor is a viable tool for simultaneous biomarker determination.
• This technology offers potential for improved early disease screening and diagnosis by analyzing multiple biomarkers concurrently.
• Simultaneous detection of these biomarkers can significantly enhance the success rate of diagnostics.