Immunomodulatory Effects of Spherical Date Seed Pills Industrially Fabricated on RAW264.7 Cells

Dates have been demonstrated to display a variety of bioactivities and are rich in polyphenols. In this work, we assessed the underlying immunomodulatory effects of date seed polyphenol extracts that had been industrially encapsulated and fabricated into commercial pills in RAW264.7 macrophages using the NF-κB and Nrf2 signaling pathways. The outcomes showed that in RAW264.7 cells, the date seed pills effectively stimulated nuclear translocation of NF-E2-related factor 2 (Nrf2) and NF-κB, along with downstream cytokines (IL-1β, TNF-α, IL-6, and IFN-γ), ROS ratios, and SOD activity. It is interesting to note that the encapsulated pills activated Nrf2 nuclear translocation more effectively than the non-encapsulated ones did. Additionally, pills at 50 µg mL^-1 improved immunological responses, but pills at 1000 µg mL^-1 prevented macrophages from becoming inflamed. These results showed that the immunomodulatory effects were differently impacted by commercial date seed pills, a finding which was related to the large-scale manufacturing of the pills and the incubation concentrations used. These results also shed light on a new trend of using food byproducts as an innovative supplement.