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OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision
Jiri Lindovsky1, Marcela Palkova1, Viktoriia Symkina1
1Czech Centre for Phenogenomics, Institute of Molecular Genetics, Czech Academy of Sciences, 252 50 Vestec, Czech Republic.
Genes
|February 25, 2023
Summary
This study adapts human eye imaging techniques, coherent optical tomography and electroretinography, for high-throughput mouse retina phenotyping. These methods establish normal retinal parameters and detect genetic disease models, aiding large-scale research.
Area of Science:
- Ophthalmology
- Genetics
- Animal Models
Background:
- High-throughput phenotyping is crucial for understanding gene function and disease.
- Assessing mouse retina morphology and function aids in modeling human eye diseases.
- Standardized imaging techniques are needed for reproducible large-scale studies.
Purpose of the Study:
- To adapt and validate coherent optical tomography and electroretinography for high-throughput mouse retina analysis.
- To establish normal retinal parameters in C57Bl/6NCrl mice across various ages.
- To demonstrate the utility of these techniques in identifying retinal pathologies in genetically modified mice.
Main Methods:
- Coherent optical tomography (OCT) for retinal structure.
- Electroretinography (ERG) for retinal function.
- Angiography for vascular complex analysis.
Main Results:
- Established normal ranges for key retinal parameters in wild-type mice (ages 10-100 weeks).
- Successfully identified mild and severe retinal pathologies in gene-knockout models.
- Demonstrated the feasibility of high-throughput data acquisition and analysis.
Conclusions:
- Coherent optical tomography and electroretinography are effective for high-throughput mouse retina phenotyping.
- These techniques can identify genetic disease models and aid in understanding gene-specific retinal dysfunction.
- The study provides a foundation for integrating these methods into large-scale initiatives like the International Mouse Phenotyping Consortium.
