Related Experiment Video

Updated: Aug 9, 2025

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers
06:02

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers

Published on: February 20, 2017

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ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer

Petr Daniel1, Kamila Balušíková1, Radka Václavíková2,3

  • 1Department of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, 100 00 Prague, Czech Republic.

Genes
|February 25, 2023
PubMed

Insights

TRIP6 gene expression in breast cancer is regulated by promoter methylation and gene amplification. Higher TRIP6 levels are found in taxane-resistant cells and specific patient groups.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Limited research exists on regulating TRIP6 expression in cancer.
  • TRIP6 expression is elevated in MCF-7 breast cancer cells and taxane-resistant sublines.

Purpose of the Study:

  • To investigate the regulation of TRIP6 expression in breast cancer cells.
  • To understand TRIP6 overexpression in taxane resistance.

Main Methods:

  • Analysis of TRIP6 promoter methylation and cyclic AMP response element (CRE) activity.
  • Fluorescence in situ hybridization (FISH) to assess gene amplification.
  • Correlation analysis with clinical data (progesterone receptor status, menopausal status).

Main Results:

  • TRIP6 transcription is primarily regulated by CRE in hypomethylated promoters in both sensitive and resistant cells.
  • TRIP6 co-amplification with ABCB1 gene observed in resistant cells, leading to overexpression.
  • High TRIP6 mRNA levels detected in progesterone receptor-positive breast cancer and premenopausal women's samples.

Conclusions:

  • TRIP6 regulation involves promoter methylation and gene amplification, particularly in taxane resistance.
  • TRIP6 overexpression is linked to specific breast cancer subtypes and patient demographics.
Keywords:
ABCB1CpG methylationMCF-7TRIP6breast cancercAMP response elementgene amplification

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