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Role of miR-143 and miR-146 in Risk Evaluation of Coronary Artery Diseases in Autopsied Samples
Jian Tie1, Hiroki Takanari2, Koya Ota1
1Department of Legal Medicine, Nihon University School of Medicine, Tokyo 1738610, Japan.
Insights
Single nucleotide polymorphisms (SNPs) in microRNA-146a (miR-146a) are associated with coronary artery disease (CAD) risk in the Japanese population. The Has-miR-143 SNP showed no clear correlation with CAD risk.
Area of Science:
- Genetics
- Cardiovascular Medicine
- Molecular Biology
Background:
- Coronary artery disease (CAD) is a leading cause of mortality worldwide.
- MicroRNA (miRNA) polymorphisms, including Has-miR-143 (rs41291957 C>G) and Has-miR-146a (rs2910164 G>A), are recognized genetic risk factors for CAD.
- Previous genetic association studies have not investigated these specific miRNA SNPs in the Japanese population.
Purpose of the Study:
- To investigate the association between single nucleotide polymorphisms (SNPs) in Has-miR-143 (rs41291957) and Has-miR-146a (rs2910164) and the risk of coronary artery disease (CAD) in Japanese individuals.
- To analyze the correlation between these miRNA genotypes and the degree of coronary artery atresia.
Main Methods:
- Utilized the TaqMan SNP assay to genotype 151 subjects with forensic autopsy-proven CAD.
- Employed ImageJ software for pathological assessment of coronary artery atresia.
- Analyzed genotype and miRNA content in relation to coronary artery atresia severity (<10% vs. >10%).
Main Results:
- The CC genotype of Has-miR-146a rs2910164 was found to be significantly more frequent in CAD patients compared to controls.
- This specific genotype (CC) of rs2910164 was associated with an increased risk of CAD in the studied Japanese population.
- No significant correlation was observed between the Has-miR-143 rs41291957 genotype and CAD risk.
Conclusions:
- Has-miR-146a rs2910164 polymorphism, specifically the CC genotype, represents a potential genetic marker for coronary artery disease risk in the Japanese population.
- Has-miR-143 rs41291957 polymorphism does not appear to be a significant genetic risk factor for CAD in this cohort.
- Further research is warranted to elucidate the precise mechanisms linking miR-146a polymorphisms to CAD pathogenesis.
Abstract:
Coronary artery disease (CAD) is a common and fatal cardiovascular disease. Among known CAD risk factors, miRNA polymorphisms, such as Has-miR-143 (rs41291957 C>G) and Has-miR-146a (rs2910164 G>A), have emerged as important genetic markers of CAD. Despite many genetic association studies in multiple populations, no study assessing the association between CAD risk and SNPs of miR-143 and miR-146 was documented in the Japanese people. Therefore, using the TaqMan SNP assay, we investigated two SNP genotypes in 151 subjects with forensic autopsy-proven CAD. After pathological observation, we used ImageJ software to assess the degree of coronary artery atresia. Moreover, the genotypes and miRNA content of the two groups of samples with atresia <10% and >10% were analyzed. The results showed that the CC genotype of rs2910164 was more frequent in patients with CAD than in controls, which was associated with the risk of CAD in the study population. However, Has-miR-143 rs41291957 genotype did not show a clear correlation with the risk of CAD.
