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Related Experiment Video

Updated: Aug 9, 2025

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Kinesins Modify ERR1-Dependent Transcription Using a Conserved Nuclear Receptor Box Motif.

A M Pramodh Bandara Seneviratne1,2, Sarah Lidagoster1, Sofia Valbuena-Castor1

  • 1CUNY School of Medicine, City College of New York, New York, NY 10031, USA.

International Journal of Molecular Sciences
|February 25, 2023
PubMed
Summary
This summary is machine-generated.

Kinesin motors regulate transcription. The kinesin-3 motor KIF1B binds estrogen related receptor alpha (ERR1) via an LxxLL motif, inhibiting ERR1-mediated transcription and suggesting expanded roles for kinesins in nuclear receptor regulation.

Keywords:
LxxLLestrogen related receptor alphakinesintranscriptional regulation

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Kinesin motors are microtubule-dependent ATPases involved in intracellular transport and cell division.
  • Some kinesins regulate transcription by interacting with nuclear receptors or DNA.
  • The LxxLL nuclear receptor box motif in kinesin KIF17 mediates binding to estrogen related receptor alpha (ERR1), suppressing its transcription.

Purpose of the Study:

  • To investigate the effects of kinesins with LxxLL motifs on ERR1-mediated transcription.
  • To determine if other kinesin motors, besides KIF17, regulate ERR1.

Main Methods:

  • Analysis of kinesin family proteins for LxxLL motifs.
  • Biochemical assays to test binding between KIF1B and ERR1.
  • Functional assays to assess the impact of KIF1B on ERR1-dependent transcription and nuclear entry.

Main Results:

  • Kinesin-3 motor KIF1B contains two LxxLL motifs, with one binding to ERR1.
  • Expression of a KIF1B fragment with an LxxLL motif inhibits ERR1-dependent transcription.
  • KIF1B regulates ERR1 nuclear entry through a mechanism distinct from KIF17.

Conclusions:

  • Kinesin KIF1B directly interacts with ERR1 and modulates its transcriptional activity.
  • Kinesins play a broader role in nuclear receptor-mediated transcriptional regulation than previously understood.
  • The LxxLL motif is a key functional domain for kinesin-nuclear receptor interactions in transcriptional control.