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Retinal Venule Coverage by Pericytes Decreases in Multiparous Mice in a Time-Dependent Manner Post-Delivery.

Junie P Warrington1, Maria Jones-Muhammad2, Rachael O Thompson1

  • 1Department of Neurology, University of Mississippi Medical Center, Jackson, MS 39216, USA.

International Journal of Molecular Sciences
|February 25, 2023
PubMed
Summary

Female reproductive history, or multiparity, impacts retinal vascular structure. Venous pericyte density decreases with time since last litter and age, suggesting time-dependent vascular changes.

Keywords:
paritypericytesretinal vasculatureretired breederssex

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Area of Science:

  • Ophthalmology
  • Cardiovascular Science
  • Reproductive Biology

Background:

  • Retinal vascular structure changes with age and is linked to cardiovascular risk.
  • Multiparous females often exhibit poorer cardiovascular health.
  • Investigating retinal vascular changes in multiparity is crucial for understanding cardiovascular health disparities.

Purpose of the Study:

  • To investigate the hypothesis that multiparity alters retinal vascular caliber in mice.
  • To compare retinal vascular structure between multiparous females, nulliparous females, and male breeders.
  • To identify potential correlations between reproductive history and retinal vascular changes.

Main Methods:

  • Utilized SMA-GFP reporter mice for retinal vascular assessment.
  • Compared age-matched nulliparous females, multiparous females (retired breeders), and male breeders.
  • Quantified retinal arteriole and venule number and diameter, and venous pericyte density.

Main Results:

  • Multiparous females showed increased body mass, heart weight, and kidney weight compared to nulliparous mice.
  • No significant differences were found in the number or diameter of retinal arterioles or venules among groups.
  • Venous pericyte density was significantly lower in multiparous mice compared to nulliparous mice and negatively correlated with time since last litter and age.

Conclusions:

  • Multiparous females exhibit altered retinal vascular structure, specifically reduced venous pericyte density.
  • The time elapsed since the last litter is a critical factor influencing these vascular changes.
  • Retinal vascular changes associated with multiparity are time- and age-dependent, warranting further investigation into functional consequences.