Nanostructure Mediated Piezoelectric Effect of Tetragonal BaTiO₃ Coatings on Bone Mesenchymal Stem Cell Shape and...

In recent years, porous titanium (Ti) scaffolds with BaTiO₃ coatings have been designed to promote bone regeneration. However, the phase transitions of BaTiO₃ have been understudied, and their coatings have yielded low effective piezoelectric coefficients (EPCs < 1 pm/V). In addition, piezoelectric nanomaterials bring many advantages in eliciting cell-specific responses. However, no study has attempted to design a nanostructured BaTiO₃ coating with high EPCs. Herein, nanoparticulate tetragonal phase BaTiO₃ coatings with cube-like nanoparticles but different effective piezoelectric coefficients were fabricated via anodization combining two hydrothermal processes. The effects of nanostructure-mediated piezoelectricity on the spreading, proliferation, and osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMSCs) were explored. We found that the nanostructured tetragonal BaTiO₃ coatings exhibited good biocompatibility and an EPC-dependent inhibitory effect on hJBMSC proliferation. The nanostructured tetragonal BaTiO₃ coatings of relatively smaller EPCs (<10 pm/V) exhibited hJBMSC elongation and reorientation, broad lamellipodia extension, strong intercellular connection and osteogenic differentiation enhancement. Overall, the improved hJBMSC characteristics make the nanostructured tetragonal BaTiO₃ coatings promising for application on implant surfaces to promote osseointegration.