Related Experiment Video
Updated: Aug 9, 2025
11:14
Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
Published on: October 2, 2016
11.7K
Fast and Accurate Identification of Candida auris by High Resolution Mass Spectrometry
Azadeh Jamalian1, Joanna Freeke1, Anuradha Chowdhary2
1Centre of Expertise in Mycology, Radboud UMC/Canisius Wilhelmina Hospital, 6532 SZ Nijmegen, The Netherlands.
Journal of Fungi (Basel, Switzerland)
|February 25, 2023
Summary
A new mass spectrometry method accurately identifies Candida auris, including its clades, aiding in tracking the spread of this emerging fungal pathogen and improving hospital infection control.
Area of Science:
- Medical Mycology
- Infectious Diseases
- Analytical Chemistry
Background:
- * *Candida auris* is an emerging multidrug-resistant fungal pathogen causing nosocomial outbreaks globally.
- * Variable antifungal resistance and hospital transmission necessitate improved diagnostic methods.
- * Current identification methods face challenges with newly emerging *C. auris* lineages.
Purpose of the Study:
- * To evaluate an innovative liquid chromatography-high resolution Orbitrap mass spectrometry method for *C. auris* identification.
- * To assess the method's accuracy and efficiency across different clades and sample types.
- * To determine the potential for clade-level identification for epidemiological surveillance.
Main Methods:
- * Analysis of 102 *Candida auris* strains from five clades using liquid chromatography-high resolution Orbitrap mass spectrometry.
- * Identification from axenic cultures and plate cultures.
- * Comparison with existing identification standards.
Main Results:
- * The mass spectrometry method achieved 99.6% identification accuracy for *C. auris* from plate cultures.
- * All investigated strains, representing all five clades, were correctly identified.
- * The method provided species identification down to the clade level, demonstrating potential for epidemiological tracking.
Conclusions:
- * Liquid chromatography-high resolution Orbitrap mass spectrometry is a rapid and accurate method for identifying *Candida auris*.
- * Clade-level identification capability supports epidemiological surveillance and differentiation of transmission events.
- * This technology offers a valuable tool for managing *C. auris* in healthcare settings.
Related Concept Videos
01:19MALDI-TOF Mass Spectrometry
5.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.0K
01:15High-Resolution Mass Spectrometry (HRMS)
1.5K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.5K
01:21Mass Spectrometry: Complex Analysis
854
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
854