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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Aug 8, 2025

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Prism Design for Spectral Flow Cytometry.

Zixi Chao¹, Yong Han¹, Zeheng Jiao¹

¹Department of Precision Instruments, Tsinghua University, Beijing 100084, China.

|February 25, 2023

Summary

This summary is machine-generated.

Prism dispersion offers a more effective and accurate method for full-spectrum fluorescence detection in flow cytometry. This study develops design criteria for prism dispersion, optimizing performance for miniaturized flow cytometers.

Keywords:

dispersion flow cytometer micro-flow cytometer prism spectrometry

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Last Updated: Aug 8, 2025

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Area of Science:

Spectroscopy
Biomedical Instrumentation
Optical Engineering

Background:

Traditional flow cytometry uses filters for fluorescence detection, limiting accuracy and effectiveness.
Full-spectrum fluorescence detection via dispersion offers superior performance.
Miniaturization of flow cytometry demands advanced optical designs.

Purpose of the Study:

To investigate prism dispersion as a suitable method for miniaturized flow cytometry.
To derive design criteria for optimizing prism dispersion systems.
To demonstrate and validate a prism dispersion fluorescence detection system for microfluidic flow cytometers.

Main Methods:

Formulas for light path and transmittance through prisms were deduced.
Design criteria for prism top and incident angles were established for optimal transmittance and dispersion.
Multi-prism systems were optimized for size and performance.
A microfluidic flow cytometer with prism dispersion was designed and tested.

Main Results:

Prism dispersion provides higher and more uniform light energy utilization, ideal for small flow cytometers.
Optimized design criteria for single and multiple prisms were obtained.
Experimental validation using microspheres and biological samples confirmed the design's effectiveness.
The developed criteria are applicable to spectral flow cytometers.

Conclusions:

Prism dispersion is a highly suitable method for full-spectrum fluorescence detection in miniaturized flow cytometry.
The derived design criteria enable the optimization of prism dispersion systems for enhanced performance.
This approach is validated for microfluidic applications and broadly applicable to spectral flow cytometry.