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Liposome Deformation Induced by Membrane-Binding Peptides

Kayano Izumi1, Chihiro Saito1, Ryuji Kawano1

  • 1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan.

Micromachines
|February 25, 2023
PubMed

Insights

Cell-penetrating peptides (CPPs) cause greater liposome deformation than antimicrobial peptides (AMPs). Differences in peptide secondary structure influence liposome shape distortion, offering control for molecular robots.

Area of Science:

  • Biophysics
  • Materials Science
  • Biochemistry

Background:

  • Liposomes are versatile vesicles with applications in drug delivery and nanotechnology.
  • Peptide-membrane interactions are crucial for understanding biological processes and designing biomimetic systems.
  • Controlling liposome architecture is key for advanced applications like molecular robotics.

Purpose of the Study:

  • To investigate how different membrane-binding peptides affect liposome deformation and shape distortion.
  • To compare the effects of cell-penetrating peptides (CPPs) and antimicrobial peptides (AMPs) on liposome integrity.
  • To explore the potential of using peptide structure to regulate liposome architecture for molecular robotics.

Main Methods:

  • Utilized fluorescent microscopy to monitor liposome deformation.
  • Employed capacitance measurements to estimate peptide binding to lipid bilayers (DOPC membranes).
  • Compared the effects of four peptides: TAT, C105Y (CPPs), melittin, and ovispirin (AMPs).

Main Results:

  • Cell-penetrating peptides (CPPs) induced significantly higher liposome deformation and shape distortion compared to antimicrobial peptides (AMPs).
  • The peptide C105Y did not cause liposome rupture, unlike TAT, melittin, and ovispirin.
  • Observed variations in liposome distortion correlated with peptide secondary structures (α-helix vs. random coil).

Conclusions:

  • Peptide secondary structure plays a critical role in dictating the extent of liposome deformation and distortion.
  • Peptides can be utilized to precisely control liposome architecture.
  • This research provides a foundation for developing peptide-mediated control of liposomal molecular robots.
Keywords:
liposome deformationmembrane capacitancepeptide

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