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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

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MEMS Enabled Miniature Two-Photon Microscopy for Biomedical Imaging.

Xiaomin Yu¹, Liang Zhou², Tingxiang Qi³

¹Key Laboratory of Biological Effect of Physical Field and Instrument, Department of Electrical and Electronic Engineering, Chengdu University of Information Technology, Chengdu 610225, China.

|February 25, 2023

Summary

Miniaturized two-photon microscopy (TPM) systems overcome the bulkiness of traditional setups. Microelectromechanical systems (MEMS) and micro-optics enable compact, high-resolution in vivo brain imaging for research and clinical use.

Keywords:

MEMS mirrors axial scanning head-mounted microendoscopy miniaturized two-photon microscopy

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Area of Science:

Neuroscience
Biomedical Engineering
Optical Imaging

Background:

Two-photon microscopy (TPM) is crucial for in vivo brain imaging, offering optical sectioning and sub-cellular resolution.
Traditional TPM systems are bulky, limiting their widespread application in research and clinical settings.
Miniaturization of TPM is essential for broader accessibility and novel applications.

Purpose of the Study:

To review recent advancements in miniaturized two-photon microscopy (TPM) systems.
To explore the integration of micro-optics and microelectromechanical systems (MEMS) for compact TPM probes.
To detail progress in both lateral and axial scanning techniques for miniaturized TPM.

Main Methods:

Review of lateral 2D scanning mechanisms, including electrostatic, electromagnetic, and electrothermal actuation in miniature TPM probes.
Review of axial scanning mechanisms, such as MEMS microlenses, remote-focus, liquid lenses, and deformable MEMS mirrors.
Analysis of the integration challenges and opportunities presented by MEMS and micro-optics for miniaturized TPM.

Main Results:

Significant progress has been made in developing miniature TPM probes with integrated scanning capabilities.
Various micro-actuation techniques enable precise lateral scanning within compact probe designs.
Advanced micro-optics provide effective axial scanning solutions for miniaturized TPM.

Conclusions:

MEMS technology and micro-optics are key enablers for creating miniaturized TPM systems.
Miniaturized TPM probes offer a promising solution to overcome the size limitations of conventional systems.
These advancements pave the way for more accessible and versatile in vivo optical brain imaging.