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Phosphate-Solubilizing Capacity of Paecilomyces lilacinus PSF7 and Optimization Using Response Surface Methodology
Xue-Li Wang1,2,3, Shu-Yi Qiu1,2,3, Shao-Qi Zhou1,2,3
1College of Life Sciences, Guizhou University, No. 2708 Huaxi Road, 14, Guiyang 550025, China.
Microorganisms
|February 25, 2023
Summary
This study identified Paecilomyces lilacinus (PSF7) as a phosphorus-solubilizing microorganism. Optimized conditions significantly enhanced its ability to solubilize phosphate, showing potential for agricultural biofertilizers.
Area of Science:
- Agricultural Microbiology
- Soil Science
- Biotechnology
Background:
- Phosphorus is crucial for plant growth but often unavailable in insoluble forms.
- Phosphorus-solubilizing microorganisms (PSMs) can convert insoluble phosphates into plant-available forms.
- Investigating novel PSMs is vital for sustainable agriculture and reducing chemical fertilizer use.
Purpose of the Study:
- To isolate and identify a novel phosphorus-solubilizing microorganism from a phosphorus tailings dump.
- To characterize the phosphorus-solubilizing capabilities of the isolate, Paecilomyces lilacinus (PSF7).
- To optimize medium components for enhanced phosphorus solubilization by PSF7.
Main Methods:
- Isolation and identification of microorganism PSF7 using morphological characterization and ITS sequencing.
- Liquid fermentation to study the relationship between phosphorus solubilization and pH variation.
- High-performance liquid chromatography (HPLC) to identify organic acids produced.
- Response surface methodology (RSM) to optimize medium components for maximum phosphorus solubilization.
- Scanning electron microscopy (SEM) to visualize microbial interaction with mineral phosphate.
Main Results:
- PSF7 was identified as Paecilomyces lilacinus.
- A significant negative correlation (r = -0.784) was observed between soluble phosphorus content and pH.
- Eight organic acids were detected, with tartaric and formic acids being predominant under low phosphorus stress.
- Optimized medium components (sucrose, ammonium sulfate, soybean residue, inorganic salts, tricalcium phosphate) predicted a soluble phosphorus content of 123.89 mg/L.
- Actual soluble phosphorus content reached 122.17 mg/L under optimized conditions.
Conclusions:
- Paecilomyces lilacinus (PSF7) demonstrates significant phosphorus-solubilizing potential.
- The study provides optimized conditions for maximizing phosphorus solubilization by PSF7.
- PSF7 shows promise as an effective biofertilizer for improving phosphorus availability in soils.
