Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

20.4K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
20.4K
Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

6.7K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
6.7K
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

107
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
107
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

157
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
157
Van de Graaff Generator01:15

Van de Graaff Generator

1.8K
Van de Graaff generators (or Van de Graaffs) are devices used to demonstrate high voltage due to static electricity that can also be used for research. Robert Van de Graaff first built one in 1931 (based on original suggestions by Lord Kelvin) for use in nuclear physics research.
Van de Graaff uses both smooth and pointed surfaces, conductors, and insulators to generate large static charges and, hence, large voltages. A substantial excess charge can be deposited on the sphere because it moves...
1.8K
Voltammetry: Factors Affecting Measurements01:21

Voltammetry: Factors Affecting Measurements

189
A current produced due to the redox reactions of the analyte at the working and auxiliary electrodes is called a faradaic current. The reaction can be divided into two types. The current generated due to the reduction of the analyte is called cathodic current, and it carries a positive charge. In contrast, the current produced by analyte oxidation is known as an anodic current, and it has a negative charge. The applied potential at the working electrode determines the faradaic current flow, and...
189

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hidden Fracture, Urgent Thrombolysis: Intravenous Alteplase for Acute Ischemic Stroke With an Occult Proximal Femoral Fracture After a Fall.

Cureus·2026
Same author

Corrigendum to: "COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities" [iScience, Volume 26, Issue 7 (2023) 107019].

iScience·2026
Same author

Intersections of evolution and ecology of polymorphisms from the viewpoint of demographic performance.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
Same author

Unexpected Air on Trauma Computed Tomography: Iatrogenic Intravascular Air After Peripheral Venous Cannulation.

Cureus·2026
Same author

Emergence of Tigecycline-Resistant Pseudomonas aeruginosa Harbouring tmexC6D6-toprJ1b From Hospital Sewage in Japan.

Environmental microbiology reports·2026
Same author

Inactivation of bio-aerosolized viruses by applying pulsed electric field.

Environmental pollution (Barking, Essex : 1987)·2026

Related Experiment Video

Updated: Aug 8, 2025

Electroporation of Mycobacteria
11:57

Electroporation of Mycobacteria

Published on: May 23, 2008

25.5K

Vancomycin-Resistant Enterococcus faecium Sterilization and Conductivity Change by Impulse Voltage.

Takahisa Ueno1, Takashi Furukawa2, Takashi Sakugawa3

  • 1Department of Electrical and Electronic Engineering, National Institute of Technology, Oita College, 1666 Maki, Oita-shi 870-0152, Oita, Japan.

Microorganisms
|February 25, 2023
PubMed
Summary

Impulse voltage effectively inactivates drug-resistant bacteria in wastewater, offering a promising alternative to chlorine sterilization. Sterilization efficiency is enhanced by higher temperatures and electrical conductivity.

Keywords:
conductivitydrug-resistant bacteriaimpulse voltagesilicon-insulated gate bipolar transistor processessterilizationvancomycin

More Related Videos

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

2.9K
Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells
10:12

Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells

Published on: September 6, 2024

342

Related Experiment Videos

Last Updated: Aug 8, 2025

Electroporation of Mycobacteria
11:57

Electroporation of Mycobacteria

Published on: May 23, 2008

25.5K
The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

2.9K
Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells
10:12

Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells

Published on: September 6, 2024

342

Area of Science:

  • Environmental microbiology
  • Water treatment technologies
  • Antimicrobial resistance

Background:

  • Antibiotic resistance, particularly vancomycin resistance, is a growing global health concern.
  • Conventional chlorine-based sewage sterilization may be insufficient to eliminate resistant bacteria.
  • There is a need for effective methods to inactivate drug-resistant bacteria in wastewater.

Purpose of the Study:

  • To investigate the efficacy of impulse voltage as a sterilization method for drug-resistant bacteria.
  • To determine the optimal conditions for impulse-voltage-mediated bacterial inactivation.
  • To explore the correlation between sterilization rate and physical parameters like temperature and conductivity.

Main Methods:

  • Application of impulse voltage to bacterial suspensions containing drug-resistant strains.
  • Varying applied voltage (above 4.5 kV) and application times.
  • Monitoring bacterial inactivation rates (CFU/mL).
  • Measuring temperature and electrical conductivity of the bacterial suspension.

Main Results:

  • Sterilization of bacteria at concentrations above 10^5 CFU/mL was achieved with impulse voltages above 4.5 kV and sufficient application time.
  • Sterilization effectiveness increased with higher temperatures of the bacterial suspension.
  • A correlation was observed between the number of sterilized bacteria and electrical conductivity when exceeding 10^5 CFU/mL.

Conclusions:

  • Impulse voltage is a viable method for inactivating drug-resistant bacteria in wastewater.
  • Sterilization efficiency is influenced by voltage, application time, temperature, and electrical conductivity.
  • Electrical conductivity can serve as a rapid indicator for estimating sterilization rates, eliminating the need for traditional culture methods.