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Detection of Copy Number Alterations Using Single Cell Sequencing
Published on: February 17, 2017
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Protoplast Preparation for Algal Single-Cell Omics Sequencing.
Junran Ye1, Cuiqiyun Yang1, Luojia Xia1
1Division of Natural and Applied Sciences, Duke Kunshan University, 8 Duke Ave, Kunshan 215316, China.
Microorganisms
|February 25, 2023
Summary
Single-cell sequencing (SCS) is powerful for life science research. This review details preparing algal protoplasts for SCS, overcoming cell wall challenges in eukaryotic algae.
Area of Science:
- Marine Biology
- Biotechnology
- Genomics
Background:
- Single-cell sequencing (SCS) offers high throughput and resolution for biological research.
- Algal cell walls impede direct application of current SCS pipelines due to RNA extraction difficulties.
- Algal protoplasts (cells without walls) are compatible with existing SCS platforms.
Purpose of the Study:
- To review algal cell wall composition, focusing on Chlorophyta.
- To summarize advancements in producing algal protoplasts via physical, chemical, and biological methods.
- To provide a guide for applying SCS in eukaryotic algal research.
Main Methods:
- Review of literature on algal cell wall structure and composition.
- Compilation of methods for algal protoplast preparation (physical, chemical, biological).
- Case studies of protoplast production in common eukaryotic algae.
Main Results:
- Detailed summary of algal cell wall characteristics, especially in Chlorophyta.
- Overview of effective protoplast isolation techniques.
- Examples of successful protoplast generation in diverse algal species.
Conclusions:
- Algal protoplast preparation is key to unlocking SCS applications in algal research.
- This review serves as a primer for researchers adapting SCS to eukaryotic algae.
- Overcoming cell wall barriers enables advanced genomic studies in algae.
