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Related Experiment Video

Updated: Aug 8, 2025

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
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GSH-Activatable Aggregation-Induced Emission Cationic Lipid for Efficient Gene Delivery.

Yue-Rui Yuan1, Qiang Liu1, Deyu Wang1

  • 1Key Laboratory of General Chemistry of the National Ethnic Affairs Commission, College of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China.

Molecules (Basel, Switzerland)
|February 25, 2023
PubMed
Summary

Researchers developed a novel lipid delivery system for gene therapy. This system uses a glutathione-activated fluorescent lipid (QM-SS-KK) for efficient DNA delivery and tracking, outperforming commercial agents.

Keywords:
aggregation-induced emissioncationic lipidsgene deliveryquinoline-malononitrilereduction responsive

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Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Nanotechnology

Background:

  • Gene therapy relies on effective and safe delivery vehicles.
  • Current nonviral gene delivery methods often face challenges with efficiency and stability, especially in biological fluids.

Purpose of the Study:

  • To design and synthesize a novel glutathione-activated, aggregation-induced-emission (AIE) cationic amphiphilic lipid (QM-SS-KK) for nonviral gene delivery.
  • To evaluate the gene delivery efficiency and tracking capabilities of liposomes formulated with QM-SS-KK.

Main Methods:

  • Synthesis of QM-SS-KK, a lipid comprising a lysine tripeptide headgroup, a disulfide linkage, and a quinoline-malononitrile (QM) AIE fluorophore tail.
  • Formation of QM-SS-KK/DOPE liposomes for plasmid DNA (pDNA) complexation and delivery.
  • Assessment of cellular uptake and transfection efficiency (TE) in vitro, including performance in the presence of serum.

Main Results:

  • QM-SS-KK effectively compacted pDNA and formed stable liposomes (QM-SS-KK/DOPE).
  • The disulfide linkage in QM-SS-KK was cleaved by glutathione (GSH), triggering AIE signals for in situ tracking.
  • QM-SS-KK/DOPE liposomes demonstrated high pDNA transfection efficiency, notably outperforming polyethyleneimine (PEI25K) in 10% serum.

Conclusions:

  • Peptide and QM-based fluorescence AIE lipids represent a promising platform for advanced gene delivery systems.
  • The developed QM-SS-KK lipid offers efficient gene delivery with built-in tracking capabilities.
  • The system's enhanced performance in serum suggests potential for in vivo applications.