Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

261
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
261
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

824
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
824
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

260
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
260
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

854
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
854
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

1.6K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Variovoracins: new glycolipopeptide biosurfactants identified by surfactant screening and genome mining.

RSC advances·2026
Same author

High-Accuracy Quantitative Nuclear Magnetic Resonance Using Improved Solvent Suppression Schemes.

Analytical chemistry·2025
Same author

Augmented kurtosis-based projection pursuit: a novel, advanced machine learning approach for multi-omics data analysis and integration.

Nucleic acids research·2025
Same author

Isolation of Caribbean Ciguatoxin-5 (C-CTX5) and confirmation of its structure by NMR spectroscopy.

Tetrahedron·2024
Same author

Characterization of a partially saturated and glycosylated apocarotenoid from wheat that is depleted upon leaf rust infection.

Gene·2024
Same author

A dually emissive MPA-CdTe QDs@N, S-GQD nanosensor for sensitive and selective detection of 4-nitrophenol using two turn-off signals.

Analytical methods : advancing methods and applications·2023
Same journal

Correction: Chen et al. Chemical Composition of <i>Litsea pungens</i> Essential Oil and Its Potential Antioxidant and Antimicrobial Activities. <i>Molecules</i> 2023, <i>28</i>, 6835.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Ruan et al. Comparison of Extraction, Isolation, Purification, Structural Characterization and Immunomodulatory Activity of Polysaccharides from Two Species of <i>Cistanche</i>. <i>Molecules</i> 2025, <i>30</i>, 4754.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Li et al. Gastrodin Ameliorates Cognitive Dysfunction in Vascular Dementia Rats by Suppressing Ferroptosis via the Regulation of the Nrf2/Keap1-GPx4 Signaling Pathway. <i>Molecules</i> 2022, <i>27</i>, 6311.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Zueva et al. Steady-State Kinetics of Enzyme-Catalyzed Hydrolysis of Echothiophate, a P-S Bonded Organophosphorus as Monitored by Spectrofluorimetry. <i>Molecules</i> 2020, <i>25</i>, 1371.

Molecules (Basel, Switzerland)·2026
Same journal

1,4-Diazatriphenylene and Its Hetero-Fused Analogs: Synthesis and Applications.

Molecules (Basel, Switzerland)·2026
Same journal

Comparative Phytochemical Studies on the Aerial Parts of <i>Teucrium davaeanum</i> Coss. and <i>Teucrium zanonii</i> Pamp.

Molecules (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Aug 8, 2025

Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers
08:42

Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers

Published on: March 17, 2015

10.7K

Analysis and Discrimination of Canadian Honey Using Quantitative NMR and Multivariate Statistical Methods.

Ian W Burton1, Mohsen Kompany-Zareh2, Sophie Haverstock1

  • 1Aquatic and Crop Resource Development, National Research Council of Canada, Halifax, NS B3H 3Z1, Canada.

Molecules (Basel, Switzerland)
|February 25, 2023
PubMed
Summary
This summary is machine-generated.

A new quantitative NMR method successfully identified 33 compounds in Canadian honey. This analysis differentiated Canadian honey from international samples using statistical methods, identifying key chemical markers.

Keywords:
food adulterationhoneymultivariate statistical analysesnuclear magnetic resonance (NMR)quantitation

More Related Videos

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements
10:25

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements

Published on: June 28, 2016

10.7K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.5K

Related Experiment Videos

Last Updated: Aug 8, 2025

Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers
08:42

Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers

Published on: March 17, 2015

10.7K
Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements
10:25

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements

Published on: June 28, 2016

10.7K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

12.5K

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Agricultural Science

Background:

  • Honey adulteration is a significant global concern.
  • Ensuring the authenticity and origin of honey is crucial for consumers and regulatory bodies.
  • Previous methods for honey analysis have limitations in accuracy and scope.

Purpose of the Study:

  • To develop and validate a quantitative Nuclear Magnetic Resonance (NMR) method for analyzing honey composition.
  • To differentiate Canadian honey samples from international honeys based on their chemical profiles.
  • To identify potential chemical markers indicative of Canadian honey origin.

Main Methods:

  • Analysis of 424 Canadian honey samples using a quantitative NMR method (700 MHz spectrometer with cryoprobe).
  • Identification and quantification of 33 characteristic honey compounds.
  • Application of multivariate statistical methods: Principal Component Analysis (PCA), Partial Least Squares Discriminant Analysis (PLS-DA), and Similarity-of-Intervals Class Modeling (SIMCA).
  • Variable selection using the replacement method.

Main Results:

  • A robust and reproducible quantitative NMR methodology was established.
  • PLS-DA and SIMCA models demonstrated reliability in distinguishing Canadian honey from honeys of diverse origins and floral types.
  • Trigonelline, proline, and to a lesser extent, ethanol were identified as potential chemical markers for differentiating Canadian from non-Canadian honey.

Conclusions:

  • The developed quantitative NMR method is effective for honey authentication and origin determination.
  • Multivariate statistical analysis provides a powerful tool for classifying honey based on chemical composition.
  • Specific compounds like trigonelline and proline can serve as reliable markers for identifying Canadian honey.