Enhanced Bioactive Potential of Functionalized Injectable Platelet-Rich Plasma

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Microbiology

Background:

• Injectable platelet-rich fibrin (iPRF) is utilized for tissue regeneration and possesses antimicrobial properties.
• Human lactoferrin (Lf) is a glycoprotein with known bioactive functions.
• Combining iPRF and Lf may enhance their therapeutic potential.

Purpose of the Study:

• To evaluate the combined antimicrobial and antibiofilm effects of iPRF functionalized with human lactoferrin (Lf).
• To assess the impact of iPRF and Lf on keratinocyte proliferation.
• To investigate the synergistic effects of iPRF-Lf combinations on bacterial growth and biofilm formation.

Main Methods:

• In vitro study using CCK8 assay to evaluate keratinocyte proliferation.
• Agar-well diffusion, broth microdilution, and crystal violet assays to test antimicrobial and antibiofilm activity.
• Testing against five reference bacterial strains, including Gram-positive and Gram-negative bacteria.

Main Results:

• iPRF and Lf combinations significantly increased keratinocyte proliferation rates at 24 and 48 hours.
• The highest proliferation was observed with 7% iPRF and 50 µg/mL Lf (102.40% ± 0.80 viability).
• iPRF demonstrated antimicrobial and antibiofilm activity, enhanced by Lf, particularly at higher concentrations.

Conclusions:

• Functionalized iPRF with human lactoferrin exhibits enhanced bioactive properties.
• The iPRF-Lf combination shows increased antimicrobial and antibiofilm potential against biofilm-producing bacteria.
• Further research is needed to elucidate the specific mechanisms underlying these enhanced bioactive properties.