Related Experiment Video
Updated: Aug 8, 2025

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
Published on: June 7, 2017
Far-UVC Radiation for Disinfecting Hands or Gloves?
Martin Hessling1, Ben Sicks1, Bernhard Lau1
1Institute of Medical Engineering and Mechatronics, Ulm University of Applied Sciences, Albert-Einstein-Allee 55, D-89081 Ulm, Germany.
Far-UVC radiation shows antimicrobial effects on hands and gloves but is less effective than alcohol disinfectants. Current regulations limit its use for frequent hand disinfection in healthcare settings.
Area of Science:
- Microbiology
- Public Health
- Biophysics
Background:
- Far-UVC radiation (200-230 nm) demonstrates potent antimicrobial properties.
- It exhibits minimal harm to human skin, suggesting potential for healthcare applications.
- This study investigates its efficacy for hand disinfection in clinical environments.
Purpose of the Study:
- To evaluate the effectiveness of far-UVC radiation for disinfecting hands and gloves.
- To compare far-UVC disinfection with conventional alcohol-based disinfectants.
- To assess the feasibility of far-UVC for routine hand hygiene in healthcare.
Main Methods:
- Microbial contamination of hands and gloves followed by exposure to 222 nm far-UVC radiation at 23 mJ/cm² and 100 mJ/cm².
- Post-irradiation colony counting on agar plates after 24-hour incubation.
- Comparative disinfection using a commercial alcohol-based hand rub.
Main Results:
- A one-log reduction in microbial colonies on hands with 23 mJ/cm² far-UVC.
- A 1.3-log reduction on contaminated gloves with 100 mJ/cm² far-UVC.
- Alcohol-based disinfectant achieved higher reductions (1.9 logs on hands, ~1 log on gloves).
Conclusions:
- Far-UVC radiation offers a considerable reduction in microbial load on hands and gloves.
- Disinfection efficacy on surfaces is lower than reported for microbial suspensions.
- Current European Union irradiation limits restrict frequent use; regulatory changes are anticipated. Disinfection of gloved hands is possible, provided glove integrity is maintained.
Related Concept Videos
Hand hygiene
Hand washing...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Handwashing II: Pre-procedure and Initial Procedure Steps
The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury. The nails must be short and clean, without nail...
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Handwashing III: During the Procedure and Post-Procedure Steps
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...

