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Levels of prodynorphin mRNA in rat dentate gyrus are decreased during hippocampal kindling
B J Morris1, M E Moneta, G ten Bruggencate
1Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, F.R.G.
Abstract:
The effect of hippocampal kindling on the levels of prodynorphin mRNA in rat hippocampus was examined by in situ hybridization using a synthetic oligonucleotide probe. Cryostat tissue sections were hybridised with a 32P-labelled 100 mer DNA probe complementary to the coding region of rat prodynorphin mRNA, and exposed to X-ray film. In rats exhibiting stage 4 seizures, the levels of prodynorphin mRNA in the dentate gyrus were dramatically reduced compared to control animals. This suggests that the development of kindling is accompanied by a reduction in the rate of synthesis of peptides derived from prodynorphin.
Insights
Hippocampal kindling significantly reduced prodynorphin mRNA levels in rat dentate gyrus. This indicates decreased synthesis of prodynorphin-derived peptides during kindling development.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Kindling is a model for epilepsy and memory.
- Prodynorphin is a precursor to opioid peptides.
Purpose of the Study:
- To investigate the impact of hippocampal kindling on prodynorphin mRNA expression in rats.
Main Methods:
- In situ hybridization was used to quantify prodynorphin mRNA levels.
- A 32P-labeled DNA probe targeted the coding region of rat prodynorphin mRNA.
Main Results:
- Stage 4 seizures in kindled rats showed a significant reduction in prodynorphin mRNA in the dentate gyrus compared to controls.
Conclusions:
- Kindling development is associated with decreased prodynorphin mRNA synthesis in the hippocampus.
- This suggests altered opioid peptide production during kindling.